不同生境下五唇兰根部可培养内生细菌多样性研究

发布时间：2018-10-25 12:27

【摘要】：兰科植物内生细菌与菌根真菌的协作对宿主植物的生长、抗病、抗逆及植物修复环境能力等具有重要意义,揭示其内生细菌多样性及与生境之间的关系有助于阐明兰科植物的适应与进化机制。本研究基于16SrDNA序列分析探讨了不同生境下东南亚特有种五唇兰根部可培养内生细菌多样性及其空间异质性。结果表明:从不同生境下五唇兰根部共分离出内生细菌59株,其中从土生型五唇兰根部分离出内生细菌45株(76.27%),从石生型五唇兰根部分离出内生细菌14株(23.73%);基于内生细菌16SrDNA序列同源性分析及构建的系统发育树显示,五唇兰根部内生细菌分属于7属,即芽孢杆菌属(Bacillus)、伯克氏菌属(Burkholderia)、草酸菌属(Pandoraea)、土壤杆菌属(Agrobacterium)、类芽孢杆菌属(Paenibacillus)、泛菌属(Pantoea)、欧文氏菌属(Erwinia),其中优势属为芽孢杆菌属,次优势属为泛菌属和伯克氏菌属;多样性分析显示,土生型五唇兰根部内生细菌群落的Shannon多样性指数大于石生型五唇兰,不同生境下五唇兰根部内生细菌群落结构差异极显著(P0.01)。土生型五唇兰根部内生细菌群落优势属为芽孢杆菌属和泛菌属,石生型五唇兰根部内生细菌群落优势属为芽孢杆菌属和伯克氏菌属。
[Abstract]:The cooperation of endophytic bacteria and mycorrhizal fungi in orchid plants is of great significance for the growth of host plants, disease resistance, stress resistance and the ability of phytoremediation. Revealing the diversity of endophytic bacteria and their relationship with habitat is helpful to clarify the adaptation and evolution mechanism of orchids. Based on 16SrDNA sequence analysis, the diversity and spatial heterogeneity of culturable endophytic bacteria in the roots of the endemic species of South East Asia in different habitats were studied. The results showed that 59 strains of endophytic bacteria were isolated from the roots of Phellodendron in different habitats. Among them, 45 strains (76.27%) of endophytic bacteria were isolated from the roots of the native and 14 (23.73%) strains of endophytic bacteria were isolated from the roots of the lithogenic type, and the phylogenetic tree was constructed based on the homology analysis of the 16SrDNA sequence of endophytic bacteria. Endophytic bacteria belong to 7 genera, that is, (Bacillus), Burke's bacteria, (Burkholderia), oxalic acid bacteria, (Pandoraea), (Agrobacterium), (Paenibacillus), (Pantoea), Irvine, (Erwinia), in which the dominant genus is Bacillus. The Shannon diversity index of endophytic bacterial community in the root of native Phellodendron was higher than that of the stone-type, and the structure of endophytic bacteria in the root of P0.01 was significantly different from that in different habitats (P0.01). The secondary dominant genera were Pan-genus and Burke's genus, and the diversity analysis showed that the Shannon diversity index of endophytic bacterial community was higher than that of lithotypic Acanthopsis. The dominant genera of endophytic bacteria community in the roots of native Phellodendron were Bacillus and pan-bacteria, and the dominant endophytic communities in the roots of lithogenic pentaphyllum were Bacillus and Burke's bacillus.
【作者单位】：海南大学园艺园林学院;热带作物种质资源保护与开发利用教育部重点实验室;
【基金】：海南省自然科学基金(312063) 海南省科技成果示范推广专项(CGSF20120001-02) 中央级公益性科研院所基本科研业务费专项资金(1630032014043)~~
【分类号】：S682.31;S182

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