金龟子绿僵菌的胞外酶与毒力相关性及应用技术研究

发布时间：2018-10-26 17:38

【摘要】：对实验室筛选保存的金龟子绿僵菌菌株M11-8-7、M7-5-7、M7-10、M6-448、M8-5-83和M8-6-291,利用Folin-酚试剂法测定了蛋白酶活性、利用DNS法测定了几丁质酶活性、利用酸碱滴定的方法测定了脂肪酶活性;以黄粉虫作为标准试虫对其进行了毒力的测定。通过对毒力和胞外酶的活性之间的相关性,发现胞外蛋白酶活性和毒力之间存在较高线性关系其方程为y=47.79-12.19x,R2=0.8978,但是几丁质酶和脂肪酶的活性和毒力之间并没有线性关系。研究筛选出M11-8-7和M7-10为酶活性高且毒力强的菌株。通过调整发酵中蒸馏水的比例,选择出致病性高、发酵效果好,且不易污染的金龟子绿僵菌M11-8-7菌株,并进行了田间应用试验。试验结果显示4500亿孢子/亩金龟子绿僵菌对桃小食心虫有着良好的致死效果。5万亿孢子/亩和10万亿孢子/亩防治花生蛴螬相对于空白对照的增产效果分别为32.98%和36.8%,防虫效果分别为56.25%和87.5%,防治效果分别为52.08%和76.77%。在田间施用动物粪肥后对金龟子绿僵菌有着一定的影响,加鸡粪和羊粪的处理使得绿僵菌防治效果不明显,但是加牛粪和清水对照处理中绿僵菌防治效果分别为66.08%和74.69%。
[Abstract]:The protease activity of Metarhizium anisopliae strains M11-8-7, M7-5-7, M7-10M6-448M8-5-83 and M8-6-291were determined by Folin- phenol reagent method. The activity of chitinase was determined by DNS and lipase activity was determined by acid-base titration. The virulence of powdery mildew as standard test was determined. Through the correlation between virulence and extracellular enzyme activity, it was found that there was a high linear relationship between extracellular protease activity and virulence. But there is no linear relationship between the activity and virulence of chitinase and lipase. M11-8-7 and M7-10 were selected as strains with high enzyme activity and strong virulence. By adjusting the proportion of distilled water in fermentation, strain M11-8-7, which has high pathogenicity, good fermentation effect and no pollution, was selected and applied in the field. The results showed that 450 billion spores / mu of Metarhizium anisopliae had a good lethal effect on Peaches. The effects of 5 trillion spores / mu and 10 trillion spores / mu on the control of peanut grub were as follows: compared with the blank control, the effects of 450 billion spores / mu and 10 trillion spores / mu on the yield of peanut white grubs were as follows: 32.98% and 36.8%, The control effect was 56.25% and 87.5%, and the control effect was 52.08% and 76.77%, respectively. There was a certain effect on Metarhizium anisopliae after application of animal manure in the field. The control effect of Metarhizium anisopliae was not obvious due to the treatment of chicken manure and sheep manure. But the control effect of Metarhizium anisopliae was 66.08% and 74.69% respectively.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S476.12

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