白腐真菌共培养对磺胺二甲基嘧啶降解的影响

发布时间：2018-10-29 17:56

【摘要】：磺胺类抗生素是全国检出频率较高的一种抗菌药物,目前对磺胺类抗生素的生物降解方面研究较少,而生物法具有无二次污染、绿色环保的优点。因此本实验对杂色云芝和黄孢原毛平革菌共培养降解磺胺二甲基嘧啶进行研究。采用高效液相色谱(HPLC)和紫外分光光度计分别分析抗生素的浓度与酶活。结果显示,平板对峙实验中两菌种间菌丝呈僵持状,种间区域漆酶酶活始终高于其他区域。两种白腐真菌液体混配4 d时可以获得具有较高漆酶酶活的粗酶液,该粗酶液在48 h内对磺胺二甲基嘧啶的降解率为25.4%,明显高于杂色云芝纯培养粗酶液的降解率(9.6%)。在加入天然助氧剂和人工助氧剂2,2'-联氨双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(简称ABTS)后,混配粗酶液的降解率分别增至49%和93.1%。种间作用可以通过增加漆酶酶活以及产生助氧剂提升抗生素降解率,因此,共培养的白腐真菌可以作为抗生素污染生物治理的主要手段之一。
[Abstract]:Sulfonamides are one of the most frequently detected antibiotics in China. At present, the biodegradation of sulfanilamide antibiotics is less studied, but the biological method has the advantages of no secondary pollution and green environmental protection. Therefore, the co-culture of sulfadimethylpyrimidine with Phanerocephalus xanthosporium and Cyanoderma versicolor was studied in this experiment. High performance liquid chromatography (HPLC) and ultraviolet spectrophotometer (UV) were used to analyze the concentration and activity of antibiotics. The results showed that the mycelium between the two species was stiff and the laccase activity in the interspecific region was always higher than that in the other regions. When the two white rot fungi were mixed for 4 days, the crude enzyme solution with higher laccase activity could be obtained. The degradation rate of sulfadimethylpyrimidine was 25.4% in 48 h. The degradation rate was significantly higher than that of pure culture crude enzyme solution (9.6%). The degradation rate of the mixed crude enzyme solution was increased to 49% and 93.1% respectively after the addition of natural and artificial oxygen-aiding agents 2H 2N-diaminobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (abbreviated as ABTS). The interspecific action can increase the activity of laccase and increase the degradation rate of antibiotics. Therefore, the co-cultured white rot fungi can be used as one of the main means of biological treatment of antibiotic pollution.
【作者单位】：郑州大学化工与能源学院;
【基金】：国家水体污染控制与治理科技重大专项(2015ZX07204-002)
【分类号】：X172;X713

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