微生物源挥发性化合物苯并噻唑对灰霉病菌的抑制效应
发布时间：2018-11-01 18:13
【摘要】：【目的】评价挥发性化合物苯并噻唑防治灰霉病的应用潜力,为进一步开发利用提供依据。【方法】采用密封盘菌丝生长速率法测定山东省73株灰霉病菌(Botrytis cinerea)对苯并噻唑的敏感性,并比较不同地区和不同表现型菌株(多菌灵抗性和敏感菌株:Car~R,Car~S;腐霉利抗性和敏感菌株:Prc~R,Prc~S;嘧霉胺抗性和敏感菌株:Pyr~R,Pyr~S;啶酰菌胺抗性和敏感菌株:Bos~R,Bos~S)间的敏感性差异;测定苯并噻唑与多菌灵、啶酰菌胺室内混用对灰霉病菌的抑制作用。通过离体黄瓜叶片接种试验验证苯并噻唑对黄瓜灰霉病的保护、治疗效果以及不同熏蒸剂量和时间对灰霉病菌致病力的影响,并用扫描电子显微镜观察苯并噻唑对灰霉菌丝形态的影响。【结果】苯并噻唑对灰霉病菌菌丝的EC50范围为0.38—1.08μL·L~(-1),均值为0.62μL·L~(-1),不同地区之间无敏感性差异;山东地区多菌灵、腐霉利、嘧霉胺、啶酰菌胺抗性和敏感灰霉病菌对苯并噻唑均比较敏感,说明这4种杀菌剂与苯并噻唑无交互抗性;在苯并噻唑0.1—3μL·L~(-1)范围内,菌丝比孢子对其更敏感;2μL·L~(-1)苯并噻唑对菌丝的抑制率高达86.12%,对芽管伸长的抑制率为55.15%,而对孢子萌发的抑制率仅为19.30%。可见,病菌不同生长阶段对此化合物的敏感性不同。苯并噻唑与多菌灵混用防治黄瓜灰霉病无增效或相加作用,两者混用时哪种药剂发挥主要抑菌作用与各组分在混用中使用的浓度和比例有关;苯并噻唑与啶酰菌胺混用有显著增效作用,其田间实际应用方式、效果以及增效机制还有待进一步明确。离体叶片试验表明,苯并噻唑对黄瓜灰霉病兼具保护和治疗防效,2μL·L~(-1)的治疗防效为91.01%,高于对照药剂啶酰菌胺300 mg·L~(-1)(78.90%),并显著高于48.25%的保护防效。苯并噻唑能够显著降低灰霉病菌菌丝的致病力,受抑制程度与苯并噻唑浓度呈正相关。扫描电镜观察发现,苯并噻唑能够使菌丝形态异常,菌丝干瘪,分枝增多,表面凹陷。【结论】苯并噻唑对山东省不同地区的灰霉病菌均有较高的毒力,适合作为防治灰霉病的熏蒸剂使用。
[Abstract]:[objective] to evaluate the application potential of benzothiazole, a volatile compound, in the control of cinerea. [methods] the sensitivity of 73 strains of Botrytis cinerea (Botrytis cinerea) to benzothiazole in Shandong Province was determined by the method of the growth rate of hermetically sealed hyphae. And compare different regions and different phenotypic strains (carbendazim resistance and sensitive strains: Car~R,Car~S; The difference of sensitivity between the resistant and sensitive strains of Pythium sp. Prc~R,Prc~S; and that of Pyr~R,Pyr~S; and Bos~R,Bos~S; The inhibitory effects of benzothiazole, carbendazim and acyl chloride on Botrytis cinerea were determined. The effects of benzothiazole on the protection of cinerea and the effects of different fumigation doses and time on the pathogenicity of cinerea were tested by inoculation of cucumber leaves in vitro. The effects of benzothiazole on the morphology of Aspergillus cinerea were observed by scanning electron microscope. [results] the EC50 range of benzothiazole on the hyphae of the fungus was 0.38-1.08 渭 L L ~ (-1), and the mean value was 0.62 渭 L L ~ (-1). There was no difference in sensitivity between different regions. Carbendazim, Pythium, pyridamine, pyridamine and susceptible cinerea were all sensitive to benzothiazole, indicating that these four fungicides had no cross resistance to benzothiazole. In the range of 0.1-3 渭 L L ~ (-1) of benzothiazole, mycelium is more sensitive than spores. The inhibition rate of 2 渭 L L ~ (-1) benzothiazole on hyphae was 86.12, the inhibition rate on tube elongation was 55.15, and the inhibition rate on spore germination was 19.30%. It can be seen that the susceptibility of bacteria to this compound is different at different growth stages. The mixture of benzothiazole and carbendazim had no synergistic or additive effect on the control of cinerea in cucumber. The main bacteriostatic effect of benzothiazole and carbendazim was related to the concentration and proportion of each component used in the mixture. The combination of benzothiazole and acylamine has a significant synergistic effect, and its practical application mode, effect and synergistic mechanism need to be further clarified. In vitro leaf tests showed that benzothiazole had both protective and therapeutic effects on cucumber grey mold. The control effect of 2 渭 L L ~ (-1) was 91.01%, which was higher than that of the control drug of 300 mg L ~ (-1) (78.90%). The protective effect was significantly higher than that of 48.25%. Benzothiazole could significantly reduce the pathogenicity of the mycelium of Botrytis cinerea, and the degree of inhibition was positively correlated with the concentration of benzothiazole. It was found by SEM that benzothiazole could make hyphal morphology abnormal, mycelium shriveled, branching increased and surface hollow. [conclusion] benzothiazole has high virulence to different areas of grey mold bacteria in Shandong Province. It is suitable for use as fumigation agent to control cinerea.
【作者单位】： 山东农业大学植物保护学院;山东农业大学山东省蔬菜病虫生物学重点实验室;山东省农药毒理与应用技术省级重点实验室;
【基金】：国家重点研发计划(2016YFD0200500)
【分类号】：S432.4
本文编号：2304699
[Abstract]:[objective] to evaluate the application potential of benzothiazole, a volatile compound, in the control of cinerea. [methods] the sensitivity of 73 strains of Botrytis cinerea (Botrytis cinerea) to benzothiazole in Shandong Province was determined by the method of the growth rate of hermetically sealed hyphae. And compare different regions and different phenotypic strains (carbendazim resistance and sensitive strains: Car~R,Car~S; The difference of sensitivity between the resistant and sensitive strains of Pythium sp. Prc~R,Prc~S; and that of Pyr~R,Pyr~S; and Bos~R,Bos~S; The inhibitory effects of benzothiazole, carbendazim and acyl chloride on Botrytis cinerea were determined. The effects of benzothiazole on the protection of cinerea and the effects of different fumigation doses and time on the pathogenicity of cinerea were tested by inoculation of cucumber leaves in vitro. The effects of benzothiazole on the morphology of Aspergillus cinerea were observed by scanning electron microscope. [results] the EC50 range of benzothiazole on the hyphae of the fungus was 0.38-1.08 渭 L L ~ (-1), and the mean value was 0.62 渭 L L ~ (-1). There was no difference in sensitivity between different regions. Carbendazim, Pythium, pyridamine, pyridamine and susceptible cinerea were all sensitive to benzothiazole, indicating that these four fungicides had no cross resistance to benzothiazole. In the range of 0.1-3 渭 L L ~ (-1) of benzothiazole, mycelium is more sensitive than spores. The inhibition rate of 2 渭 L L ~ (-1) benzothiazole on hyphae was 86.12, the inhibition rate on tube elongation was 55.15, and the inhibition rate on spore germination was 19.30%. It can be seen that the susceptibility of bacteria to this compound is different at different growth stages. The mixture of benzothiazole and carbendazim had no synergistic or additive effect on the control of cinerea in cucumber. The main bacteriostatic effect of benzothiazole and carbendazim was related to the concentration and proportion of each component used in the mixture. The combination of benzothiazole and acylamine has a significant synergistic effect, and its practical application mode, effect and synergistic mechanism need to be further clarified. In vitro leaf tests showed that benzothiazole had both protective and therapeutic effects on cucumber grey mold. The control effect of 2 渭 L L ~ (-1) was 91.01%, which was higher than that of the control drug of 300 mg L ~ (-1) (78.90%). The protective effect was significantly higher than that of 48.25%. Benzothiazole could significantly reduce the pathogenicity of the mycelium of Botrytis cinerea, and the degree of inhibition was positively correlated with the concentration of benzothiazole. It was found by SEM that benzothiazole could make hyphal morphology abnormal, mycelium shriveled, branching increased and surface hollow. [conclusion] benzothiazole has high virulence to different areas of grey mold bacteria in Shandong Province. It is suitable for use as fumigation agent to control cinerea.
【作者单位】： 山东农业大学植物保护学院;山东农业大学山东省蔬菜病虫生物学重点实验室;山东省农药毒理与应用技术省级重点实验室;
【基金】：国家重点研发计划(2016YFD0200500)
【分类号】：S432.4
【相似文献】
相关期刊论文 前1条
1 刘洁;宋功武;;3-乙基苯并噻唑螺萘并VA嗪与牛血清白蛋白的相互作用[J];化学研究;2013年06期
，本文编号：2304699
本文链接：https://www.wllwen.com/kejilunwen/nykj/2304699.html
