甜瓜连作土壤中酚酸类物质测定及降解研究
发布时间：2018-12-30 18:37
【摘要】：以甜瓜连作土壤为研究对象,采用高效液相色谱技术,分析了不同连作年限甜瓜土壤中酚酸物质的种类及其含量变化,利用生防木霉菌降解特性,降解土壤中酚酸类物质,为缓解甜瓜连作障碍提供参考依据。结果表明:连作甜瓜土壤中对羟基苯甲酸、香草酸、丁香酸、香兰素、香豆酸、阿魏酸、苯甲酸随着连作年限的增加而增加,连作3年后7种酚酸的总量达245.39mg·kg~(-1)干土,显著高于未连作土壤;采用孢子菌悬液、PD菌悬液和麦麸发酵物3种不同的接种方式,测定木霉菌T23对甜瓜根际土壤中酚酸类物质的降解作用,表明木霉菌T23能够有效降解土壤中积累的酚酸物质,其中麦麸发酵物降解率可达到25%以上,降解效果最为显著。
[Abstract]:The phenolic acids in muskmelon soil under continuous cropping were analyzed by high performance liquid chromatography (HPLC). The phenolic acids were degraded by Trichoderma biocontrol. To provide reference basis for alleviating the obstacle of continuous cropping of muskmelon. The results showed that p-hydroxybenzoic acid, vanillic acid, eugenic acid, vanillin, coumaric acid, ferulic acid and benzoic acid increased with the increase of continuous cropping time. After 3 years of continuous cropping, the total amount of 7 phenolic acids reached to 245.39mg kg~ (-1) dry soil, which was significantly higher than that of non-continuous cropping soil. The degradation of phenolic acids in muskmelon rhizosphere soil by Trichoderma T23 was determined by three different inoculation methods: spore suspension, PD suspension and wheat bran fermentation. The results showed that Trichoderma T23 could effectively degrade the phenolic acid accumulated in the soil. The degradation rate of wheat bran fermentation was more than 25%, and the degradation effect was the most remarkable.
【作者单位】： 沈阳农业大学植物保护学院;中国科学院沈阳应用生态研究所;
【基金】：公益性行业(农业)科研专项经费资助项目(201503110-04)
【分类号】：S652;S153
本文编号：2395977
[Abstract]:The phenolic acids in muskmelon soil under continuous cropping were analyzed by high performance liquid chromatography (HPLC). The phenolic acids were degraded by Trichoderma biocontrol. To provide reference basis for alleviating the obstacle of continuous cropping of muskmelon. The results showed that p-hydroxybenzoic acid, vanillic acid, eugenic acid, vanillin, coumaric acid, ferulic acid and benzoic acid increased with the increase of continuous cropping time. After 3 years of continuous cropping, the total amount of 7 phenolic acids reached to 245.39mg kg~ (-1) dry soil, which was significantly higher than that of non-continuous cropping soil. The degradation of phenolic acids in muskmelon rhizosphere soil by Trichoderma T23 was determined by three different inoculation methods: spore suspension, PD suspension and wheat bran fermentation. The results showed that Trichoderma T23 could effectively degrade the phenolic acid accumulated in the soil. The degradation rate of wheat bran fermentation was more than 25%, and the degradation effect was the most remarkable.
【作者单位】： 沈阳农业大学植物保护学院;中国科学院沈阳应用生态研究所;
【基金】：公益性行业(农业)科研专项经费资助项目(201503110-04)
【分类号】：S652;S153
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