矮生嵩草内生细菌264AY1的鉴定及其生物功能测定

发布时间：2019-01-18 18:44

【摘要】：为了开发利用高寒草地优势牧草的内生细菌资源,对分离自矮生嵩草(Kobresia humilis)叶片的内生细菌264AY1进行了形态观察和16SrDNA序列鉴定,并测定了其生物功能。结果表明,菌株264AY1为革兰氏阳性菌,菌体杆状,大小为1.637~4.128×0.614~14.869μm,结合16SrDNA基因序列同源性分析,初步鉴定为枯草芽孢杆菌(Bacillus subtilis)。该菌对马铃薯炭疽病菌(Colletotrichumcoccodes)、马铃薯坏疽病菌(Phomafoveata)和马铃薯枯萎病菌(Fusariumavenaceum)的抑菌率分别为81.11%、73.1%和65.46%,并且对菌丝具有致畸作用;在含有和不含色氨酸的培养基上分泌IAA的量分别为16.05mg/L和10.75mg/L;在Pikovaskaia培养液中的有效磷增量为5.34mg/L,且具有固氮能力。该结果表明矮生嵩草内生细菌264AY1有开发为药肥兼效的微生物制剂的潜力。
[Abstract]:In order to exploit the endophytic bacteria resources of dominant forage grass in alpine grassland, the morphological observation and 16SrDNA sequence identification of endophytic bacteria 264AY1 isolated from the leaves of Kobresia humilis (Kobresia humilis) were carried out, and its biological function was determined. The results showed that the strain 264AY1 was gram-positive, and its size was 1.637 ~ 4.128 脳 0.614 ~ 14.869 渭 m. The strain was identified as Bacillus subtilis (Bacillus subtilis). By homology analysis of 16SrDNA gene sequence. The inhibitory rates of this strain on (Colletotrichumcoccodes), (Phomafoveata) and (Fusariumavenaceum) were 73.1% and 65.46%, respectively, and had teratogenic effect on mycelium. The amount of IAA secreted on the medium containing and without tryptophan was 16.05mg/L and 10.75 mg / L, respectively, and the increment of available phosphorus in Pikovaskaia medium was 5.34 mg / L, and the nitrogen fixation ability was also observed. The results showed that the endophytic bacteria 264AY1 of Kobresia humilis had the potential to develop microbial agents which were both effective and effective.
【作者单位】：甘肃农业大学/甘肃省草业工程实验室/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心;
【基金】：国家自然科学基金(31160122) 草业生态系统教育部重点实验室(甘肃农业大学)开放课题项目(CYzs-2011011)
【分类号】：S54;S182

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