柠条根际产铁载体促生菌的分离鉴定及其促生特性
发布时间：2019-01-21 15:09
【摘要】：以柠条根际土壤为试材,采用CAS(铬天青S)选择性培养基分离筛选具有产铁载体功能的根际促生菌株,通过16S r DNA序列分析进行菌株鉴定,并测定菌株解磷、固氮、产IAA(吲哚乙酸)和ACC(1-氨基环丙烷-1-羧酸)脱氨酶等促生活性,通过盆栽试验分析验证菌株Fe11对柠条幼苗生长的影响。结果表明:共分离出14株产铁载体根际促生菌,其中6株菌具备较强的产铁载体能力。所有菌株的16S r DNA序列同源性与假单胞菌达到99%~100%,假单胞菌属细菌是柠条根际产铁载体的优势菌群。多数菌株兼具2种以上促生活性,菌株Fe9、Fe11和Fe13表现出多种促生潜能。回接试验证实,接种菌株Fe11使柠条幼苗的株高、根长、叶片数、鲜质量和干质量分别增长17.25%、6.68%、17.75%、42.51%和20.67%,表明菌株Fell对柠条的生长具有良好的促进效果。
[Abstract]:The rhizosphere soil of Caragana korshinskii was used to isolate and screen the rhizosphere growth-promoting strains with the function of iron producing carrier by CAS (Chromium-azurol S) selective medium. The strains were identified by 16s r DNA sequence analysis, and the phosphorus and nitrogen fixation were determined. IAA (indoleacetic acid) and ACC (1-aminocyclopropane-1-carboxylic acid) deaminase were produced. The effects of strain Fe11 on the growth of Caragana korshinskii seedlings were tested by pot experiment. The results showed that 14 strains of iron-producing carriers were isolated and 6 of them had strong ferric carrier capacity. The homology of 16s r DNA sequence between all strains and Pseudomonas was 990.The genus Pseudomonas was the dominant carrier for iron production in the rhizosphere of Caragana korshinskii. Most strains had two or more probiotic activities, and Fe9,Fe11 and Fe13 showed a variety of probiotic potential. The results showed that the plant height, root length, leaf number, fresh weight and dry mass of Caragana seedlings increased 17.25%, 6.68%, 17.75% and 20.67%, respectively, by inoculating strain Fe11. The results showed that the strain Fell had a good promoting effect on the growth of Caragana korshinskii.
【作者单位】： 宁夏大学生命科学学院;
【基金】：国家自然科学基金资助项目(31760027) 宁夏自然科学基金资助项目(NZ1601) 宁夏大学研究生创新资助项目(GIP201612)
【分类号】：S714.3;S793.3
本文编号：2412767
[Abstract]:The rhizosphere soil of Caragana korshinskii was used to isolate and screen the rhizosphere growth-promoting strains with the function of iron producing carrier by CAS (Chromium-azurol S) selective medium. The strains were identified by 16s r DNA sequence analysis, and the phosphorus and nitrogen fixation were determined. IAA (indoleacetic acid) and ACC (1-aminocyclopropane-1-carboxylic acid) deaminase were produced. The effects of strain Fe11 on the growth of Caragana korshinskii seedlings were tested by pot experiment. The results showed that 14 strains of iron-producing carriers were isolated and 6 of them had strong ferric carrier capacity. The homology of 16s r DNA sequence between all strains and Pseudomonas was 990.The genus Pseudomonas was the dominant carrier for iron production in the rhizosphere of Caragana korshinskii. Most strains had two or more probiotic activities, and Fe9,Fe11 and Fe13 showed a variety of probiotic potential. The results showed that the plant height, root length, leaf number, fresh weight and dry mass of Caragana seedlings increased 17.25%, 6.68%, 17.75% and 20.67%, respectively, by inoculating strain Fe11. The results showed that the strain Fell had a good promoting effect on the growth of Caragana korshinskii.
【作者单位】： 宁夏大学生命科学学院;
【基金】：国家自然科学基金资助项目(31760027) 宁夏自然科学基金资助项目(NZ1601) 宁夏大学研究生创新资助项目(GIP201612)
【分类号】：S714.3;S793.3
【相似文献】
相关期刊论文 前6条
1 王敬国;植物根系和根际微生物对氮的竞争[J];土壤;1993年05期
2 姚其华,赵书军,杨利,龙成凤;根际养分活化与吸收过程的定量模拟研究进展[J];热带亚热带土壤科学;1997年02期
3 高婷;;沙蒿根际、非根际微生物数量的动态变化研究[J];宁夏农林科技;2006年03期
4 杨玉盛,俞新妥,邱仁辉,傅瑞树,陈光水;不同栽杉代数根际土壤肥力及生物学特性变化[J];应用与环境生物学报;1999年03期
5 高文星;张莉丽;任伟;马斌;周志宇;;河西走廊盐渍土不同种植年限苜蓿根际磷含量变异特征[J];草业科学;2008年07期
6 田呈明,刘建军,梁英梅,刘永华;秦岭火地塘林区森林根际微生物及其土壤生化特性研究[J];水土保持通报;1999年02期
相关会议论文 前1条
1 郑毅;张福锁;;土壤结构和耕作对根际微生态系统的影响[A];第八届全国青年土壤暨第三届全国青年植物营养与肥料科学工作者学术讨论会论文集[C];2002年
相关博士学位论文 前1条
1 胡静;放牧对典型草原优势植物大针茅根际土壤养分和根际微生物的影响[D];中国农业科学院;2015年
相关硕士学位论文 前2条
1 丁士明;稀土对土壤矿质营养及根际矿质离子迁移、吸收的影响[D];安徽农业大学;2002年
2 刘朔;三倍体毛白杨与草复合模式根际养分动态[D];四川农业大学;2006年
，本文编号：2412767
本文链接：https://www.wllwen.com/kejilunwen/nykj/2412767.html
