红树植物角果木内生真菌Penicillium sp.J54的次生代谢产物研究

发布时间：2019-02-16 08:58

【摘要】：目的红树植物角果木内生真菌Penicillium sp.J54大米固体培养基发酵的次生代谢产物的研究。方法采用多种色谱技术进行分离纯化,并根据化合物的理化常数、核磁和质谱数据进行结构鉴定;通过滤纸片琼脂扩散法进行体外抗菌活性测试;通过Ellman比色法进行乙酰胆碱酯酶抑制活性测试。结果从Penicillium sp.J54的大米固体培养基发酵产物中分离得到了6个化合物,分别鉴定为methyl 2-hydroxy-2-(5-hydroxy-2,7-dimethyl-4-oxo-4H-chromen-3-yl)acetate(1)、coniochaetone B(2)、dihydrocitrinone(3)、asperbiphenyl(4)、erythro-11α-hydroxyneocyclocitrinol(5)和麦角甾-5,7,22-三烯-3β-醇(6)。活性测试结果表明化合物3,4和6对烟草青枯病原菌具有微弱的抑制活性,而化合物2则对乙酰胆碱酯酶有较强的抑制活性。结论化合物1为新化合物,4为首次从青霉菌中分离得到。
[Abstract]:Objective to study the secondary metabolites of mangrove plant hornwood endophytic fungus Penicillium sp.J54 rice fermentation on solid medium. Methods A variety of chromatographic techniques were used to separate and purify the compounds, and their structures were identified according to their physicochemical constants, NMR and MS data, and the antibacterial activity in vitro was tested by filter paper Agar diffusion method. The inhibitory activity of acetylcholinesterase was measured by Ellman colorimetry. Results six compounds were isolated from Penicillium sp.J54 solid medium and identified as methyl 2-hydroxy-2- (5-hydroxy-2-dimethyl-4-oxo-4H-chromen-3-yl) acetate (1), coniochaetone B (2). Dihydrocitrinone (3), asperbiphenyl (4), erythro-11 伪 -hydroxyneocyclocitrinol (5) and ergosterol 722 triene-3 尾 -alcohol (6). The results of activity test showed that compounds 3O4 and 6 had weak inhibitory activity against tobacco wilt pathogens, while compound 2 had strong inhibitory activity on acetylcholinesterase. Conclusion compound 1 is a new compound and 4 is isolated from Penicillium for the first time.
【作者单位】：中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;
【基金】：国家自然科学基金(No.41406083和No.41506096) 海南省科技兴海专项资助(No.XH201410)
【分类号】：S476.1

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