小菜蛾传染性软腐病病毒的全基因组序列及其进化分析

发布时间：2019-02-28 11:04

【摘要】：传染性软腐病病毒(Iflavirus)属于小核糖核酸病毒目,是一类单股正链RNA病毒,其家族成员正在不断扩大。Iflavirus的寄主包括鳞翅目、膜翅目和半翅目的昆虫以及瓦螨,因其对一些重要的农业害虫产生致病性,因此是重要的潜在生防病毒种类之一。本研究利用小菜蛾转录组测序数据拼接出一条Iflavirus病毒基因组序列。通过查找ORF和序列相似性比对发现此基因组可编码一条完整的病毒多聚蛋白并与其他Iflavirus病毒同源,因此将这一新病毒命名为小菜蛾传染性软腐病病毒(Diamondback moth iflavirus),英文简称DBMIV。通过多聚蛋白序列与RNA聚合酶序列构建的进化树表明DBMIV与PNV、EOPLV和SEIV2进化关系最近。最后,利用RT-PCR扩增出DBMIV的外壳蛋白区域验证了新命名病毒在田间小菜蛾种群的感染。DBMIV的鉴定与命名为利用高通量测序数据鉴定病毒新种提供参考,为利用病毒生防重要蔬菜害虫小菜蛾提供研究思路。
[Abstract]:Infectious soft rot virus (Iflavirus) belongs to the small ribonucleic acid virus order, is a single strand positive chain RNA virus, and its family members are expanding. Iflavirus hosts include Lepidoptera, Hymenoptera and Hemiptera insects as well as Var mites. Because of its pathogenicity to some important agricultural pests, it is one of the important potential biocontrol viruses. In this study, a genome sequence of Iflavirus virus was obtained by sequencing data of Plutella xylostella transcript set. By searching for ORF and sequence similarity comparison, it was found that this genome could encode a complete viral polymeric protein and be homologous to other Iflavirus viruses. Therefore, the new virus was named (Diamondback moth iflavirus), of Plutella xylostella Infectious soft Rot virus (DBMIV. for short). The phylogenetic tree constructed by Polymeric protein sequence and RNA polymerase sequence showed that DBMIV had the closest evolutionary relationship with PNV,EOPLV and SEIV2. Finally, the coat protein region of DBMIV was amplified by RT-PCR to verify the infection of the newly named virus in the population of Plutella xylostella in the field. The identification and naming of DBMIV provided a reference for the identification of new species by using high-throughput sequencing data. In order to use virus biocontrol of important vegetable pest Plutella xylostella to provide research ideas.
【作者单位】：广东省生物资源应用研究所广东省动物保护与资源利用重点实验室广东省野生动物保护与利用公共实验室;
【基金】：国家自然科学基金(31371932) 广东省科学院青年科学研究基金(qnjj201602);广东省科学院科技发展专项(2017GDASCX-0107)
【分类号】：S476.13

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