云南新产区杨梅枝枯的病原菌鉴定及土壤营养元素分析

发布时间：2019-03-26 10:45

【摘要】：为了明确云南新产区杨梅枝枯的原因,利用科赫氏法则确定了引起枝枯的病原菌,依据形态学特征,结合ITS序列分析对病原菌进行鉴定,并且对发病定植苗及其容器苗、健康定植苗和本地生长的杨梅树土壤元素含量进行了分析。结果表明:引起云南杨梅枝枯的病原菌为异色拟盘多毛孢菌(Pestalotiopsis versicolor);果园内的发病定植苗与健康定植苗相比,磷、硼、钾、钙、锌元素含量都显著升高,升高幅度在55.8%~511.5%,氮的含量也略有升高(9.2%),说明果园内的定植苗处于一种相对富营养的土壤环境;与本地生长的健康杨梅树土壤相比,容器内的植株土壤磷、钾含量大大升高,升高幅度为203.2%~431.6%,镁的含量也略有升高(27.6%),其他营养元素含量都显著降低,降低幅度为28.6%~96.9%,表明容器苗的土壤相对本地生长的健康杨梅树来说,各元素含量不太均衡。该研究结果可为指导云南杨梅新产区的病害防控提供依据。
[Abstract]:In order to clarify the cause of withered branch of Myrica rubra in a new producing area of Yunnan, the pathogenic bacteria were determined by Koch's rule, and the pathogen was identified according to morphological characteristics and ITS sequence analysis, and the pathogenic colonized seedlings and their container seedlings were identified. The contents of elements in the soil of healthy colonized seedlings and locally grown Myrica rubra trees were analyzed. The results showed that the pathogen causing withered branch of Myrica yunnanensis was (Pestalotiopsis versicolor);. Compared with healthy seedlings, the contents of P, B, K, Ca and Zn in the orchard were significantly increased by 55.8% and 511.5%, and the content of nitrogen was also slightly increased (9.2%), and the contents of P, B, K, Ca and Zn in the orchard were significantly higher than those of the healthy seedlings (P, B, K, Ca, Zn). The results showed that the seedlings in the orchard were in a relatively eutrophic soil environment. Compared with the locally grown healthy Myrica rubra soil, the contents of phosphorus and potassium in the container soil increased by 203.2% and 431.6%, and the magnesium content also slightly increased (27.6%). The contents of other nutrient elements were significantly decreased by 28.6% / 96.9%, indicating that the contents of each element in the soil of container seedlings were not well balanced compared with those of locally grown healthy Myrica rubra. The results of this study can be used to guide the disease control in the new producing area of Myrica rubra in Yunnan.
【作者单位】：浙江师范大学化学与生命科学学院;浙江省农业科学院园艺研究所;
【基金】：浙江省重大科技专项重点农业项目(2012C12009-5) 公益性行业(农业)科研专项(201203089)
【分类号】：S436.67;S158

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