岩性对喀斯特灌丛土壤固氮菌与丛枝菌根真菌群落结构及丰度的影响

发布时间：2019-04-09 19:32

【摘要】：运用末端限制性片段长度多态性(terminal restriction fragment length polymorphism,T-RFLP)和荧光定量PCR(real-time PCR)法,检测喀斯特灌丛生态系统中不同岩性条件下土壤固氮菌与丛枝菌根(arbuscular mycorrhizal,AM)真菌群落结构与丰度的变化,揭示岩性对灌丛生态系统土壤中固氮菌与AM真菌群落结构与丰度的影响.结果表明不同岩性条件下,土壤固氮菌与AM真菌丰度存在显著差异,其中,固氮菌与AM真菌丰度在石灰岩土壤中最大,白云岩土壤中最小,石灰岩-白云岩夹层土壤介于两者之间;同样,不同岩性土壤中固氮菌与AM真菌群落结构存在显著性差异.土壤Olsen-P、有机碳、黏粒含量与固氮菌丰度存在显著正相关关系,而土壤全氮、黏粒含量与AM真菌丰度存在显著正相关.RDA分析表明,植物均匀度影响固氮菌群落组成结构,而植物均匀度、香农多样性指数及丰富度指数影响AM真菌群落组成结构.以上的研究结果表明:岩性主要是通过影响植物与土壤养分来影响土壤固氮菌与AM真菌群落组成结构及丰度.
[Abstract]:Using end restriction fragment length polymorphism (terminal restriction fragment length polymorphism,T-RFLP) and fluorescence quantitative PCR (real-time PCR) to detect soil nitrogen fixation bacteria and arbuscular mycorrhizal (arbuscular mycorrhizal, in karst shrub ecosystem under different lithological conditions. The change of community structure and abundance of AM) fungi revealed the influence of lithology on the community structure and abundance of nitrogen-fixing bacteria and AM fungi in shrub ecosystem soil. The results showed that the abundance of nitrogen-fixing bacteria and AM fungi were significantly different under different lithological conditions, in which the abundance of nitrogen-fixing bacteria and AM fungi was the highest in limestone soil and the smallest in dolomite soil. The limestone-dolomite interlayer soil is between the two; Similarly, there were significant differences in community structure between nitrogen-fixing bacteria and AM fungi in soils of different lithology. There was a significant positive correlation between soil Olsen-P, organic carbon, clay content and nitrogen-fixing bacteria abundance, while that between soil total nitrogen, clay content and AM fungal abundance. RDA analysis showed that plant evenness affected the composition of nitrogen-fixing bacteria community, and there was a significant positive correlation between soil total nitrogen, clay content and soil nitrogen-fixing bacteria abundance. Plant evenness, Shannon diversity index and richness index affected the composition of AM fungal community. The results indicated that lithology mainly influenced the composition and abundance of soil nitrogen-fixing bacteria and AM fungi communities by affecting plant and soil nutrients.
【作者单位】：中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室;中国地质科学院岩溶地质研究所国土资源部广西壮族自治区岩溶动力学重点实验室;
【基金】：国家重点研发计划项目(2016YFC0502404) 广西自然科学基金项目(2016GXNSFBA380179) 国家自然科学基金项目(41301273,31270551) 岩溶地质研究所基本科研业务费项目(2016004) 中国科学院“西部之光”人才培养计划项目中国地质大调查项目(121201237000160002)
【分类号】：S154.3

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