反相高效液相色谱法测定湿地根际土壤11种有机酸

发布时间：2019-04-22 11:21

【摘要】：研究了反相高效液相色谱法同时测定湿地植物根际土壤溶液中11种低分子有机酸的优化方法。探索了柱温、波长、流速、流动相中的pH值以及有机改良剂对检测效果的影响,并进一步验证方法的精密度、加标回收率、检测限等指标。结果表明,采用Waters Atlantis T3 C18(4.6×250 mm,5μm)色谱柱,在柱温为35℃,紫外检测波长210 nm,流动相为磷酸盐缓冲液(0.01 mol/L,pH 2.5)与乙腈(ACN)混合溶液,在流速0.7 m L/min时,进行梯度洗脱分离。该方法加标回收率在95.6%~103.9%,相对标准偏差在0.3%~5.2%,各酸峰面积与质量浓度线性关系良好(R2≥0.999)。该方法成功用于介质复杂的环境样品中低分子有机酸的检测,2014年4月采集的2种湿地挺水植物根际与非根际土壤溶液检测主要存在草酸与丙二酸。芦苇土壤溶液中草酸总计15.83 mg/L,丙二酸总计18.10 mg/L;扁秆呰草土壤溶液中草酸17.90 mg/L,丙二酸总计17.05 mg/L。2种植物中,根际土壤溶液中丙二酸含量均高于非根际土壤溶液。
[Abstract]:An optimized method for simultaneous determination of 11 low molecular weight organic acids in rhizosphere soil solutions of wetland plants by reversed phase high performance liquid chromatography (RP-HPLC) was studied. The effects of column temperature, wavelength, flow rate, pH value in mobile phase and organic improver on the detection effect were explored. The precision, recovery rate and detection limit of the method were further verified. The results showed that a Waters Atlantis T3 C18 (4.6 脳 250 mm, 5 渭 m) column was used, the column temperature was 35 鈩，

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