一株高效凤眼莲脱胶菌的筛选及鉴定
发布时间：2019-05-21 20:21
【摘要】：预处理是生物质能源产品制备中最昂贵、最关键的环节,生物预处理因环境友好和能耗低而受到广泛关注。为获得较好的生物预处理菌株,以脱胶效率为出发点筛选菌株,从凤眼莲(Eichhornia crassipes)生长环境介质中分离菌株,通过富集培养、刚果红平板染色法初筛,获得一株对凤眼莲脱胶效果较好的A_1菌株。在最佳条件下A_1菌株分泌的果胶酶活性、木聚糖酶活性和纤维素酶活性分别为3 925.00、8 331.67和4 883.62 nmol·s~(-1)·mL~(-1);A_1菌株对凤眼莲的1次脱胶减重率为33.37%,3次连续脱胶的累计减重率为49.26%。通过16S rRNA序列鉴定,构建系统发育树分析,该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。
[Abstract]:Pretreatment is the most expensive and critical link in the preparation of biomass energy products. Biological pretreatment has been widely concerned because of its friendly environment and low energy consumption. In order to obtain a better biological pretreatment strain, the strains were screened from the growth environment of Eichhornia crassipes (Eichhornia crassipes) according to the degumming efficiency. The strains were isolated from the growth environment of Eichhornia crassipes. The strains were screened by enrichment culture and Congo red plate staining. A strain A 鈮，
本文编号：2482365
[Abstract]:Pretreatment is the most expensive and critical link in the preparation of biomass energy products. Biological pretreatment has been widely concerned because of its friendly environment and low energy consumption. In order to obtain a better biological pretreatment strain, the strains were screened from the growth environment of Eichhornia crassipes (Eichhornia crassipes) according to the degumming efficiency. The strains were isolated from the growth environment of Eichhornia crassipes. The strains were screened by enrichment culture and Congo red plate staining. A strain A 鈮，
本文编号：2482365
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