玄参与烟草间作对土壤细菌群落结构的影响

发布时间：2019-05-26 20:59

【摘要】：为研究玄参与烟草不同种植模式下对土壤细菌群落结构的影响,采用Illumina MiSeq技术测定土壤细菌,结果表明,玄参土壤细菌的丰富度和多样性高于植烟土壤,但未达显著水平,间作有降低植烟土壤细菌丰富度和多样性而提高玄参土壤细菌多样性的趋势。作物和种植模式在门和属水平上都影响着细菌丰度及优势菌排序,门水平上,植烟土壤中Saccharibacteria显著高于玄参土壤,Gemmatimonadetes在玄参土壤中显著高于植烟土壤,Bacteroidetes在不同种植模式间差异显著;属水平上unclassified Xanthomonadaceae,norank Saccharibacteria,uncultured Gaiellales,norank SC-I-84等植烟土壤中显著高于玄参土壤,norank GR-WP33-30,norank JG30a-KF-32,Gemmatimonas,Candidatus_Solibacter,uncultured Nitrosomonadaceae,norank C0119,norank Subgroup_6,norank DA101_soil_group、norank BIrii41,Anaeromyxobacter,unclassified Xanthomonadales,uncultured Methylophilaceae,norank JG37-AG-20等玄参土壤中显著高于植烟土壤,而几乎没有菌属受种植模式影响达显著水平。主成分分析也表明作物较种植模式影响更大。因此,土壤细菌群落结构均受作物和种植模式影响,但作物影响更大,存在明显作物效应;间作土壤因作物而具有微生物群落二元结构。
[Abstract]:In order to study the effect of Xuan participating tobacco on soil bacterial community structure under different planting patterns, soil bacteria were determined by Illumina MiSeq. The results showed that the richness and diversity of soil bacteria in Panax quinquefolium soil were higher than those in tobacco planting soil, but did not reach a significant level. Intercropping has the trend of reducing bacterial richness and diversity in tobacco planting soil and increasing bacterial diversity in Panax quinquefolium soil. The abundance of bacteria and the order of dominant bacteria were affected by crop and planting patterns at both door and genus level. at the gate level, Saccharibacteria in tobacco-growing soil was significantly higher than that in Panax quinquefolium soil, and Gemmatimonadetes was significantly higher in Panax quinquefolium soil than in tobacco-growing soil. There were significant differences in Bacteroidetes among different planting patterns. At the genus level, unclassified Xanthomonadaceae,norank Saccharibacteria,uncultured Gaiellales,norank SC-I-84 and other tobacco planting soils were significantly higher than those of Panax quinquefolium, norank GR-WP33-30,norank JG30a-KF-32,Gemmatimonas,Candidatus_Solibacter,uncultured Nitrosomonadaceae,norank C0119, Norank Subgroup_6,norank DA101_soil_group,norank BIrii41,Anaeromyxobacter,. The soil of Panax quinquefolium such as unclassified Xanthomonadales,uncultured Methylophilaceae,norank JG37-AG-20 was significantly higher than that of tobacco planting soil, and almost no genus was significantly affected by planting pattern. Principal component analysis also showed that crops had a greater influence than planting patterns. Therefore, the structure of soil bacterial community is affected by crop and planting pattern, but the influence of crop is greater, and there is obvious crop effect, and the intercropping soil has the binary structure of microbial community because of crop.
【作者单位】：西南大学资源环境学院;贵州省烟草公司遵义市公司;重庆市中药研究院;
【基金】：贵州省烟草公司遵义市公司研究开发项目(201404) 国家烟草专卖局重点项目(NY20150601070012) 中央高校基本科研业务费专项资金(SWU113094) 国家自然科学基金(31370602) 重庆市科委“科技平台与基地建设”项目(CSTC,2014ptyjd10001)资助
【分类号】：S154.3

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