辐照对瓜实蝇遗传区性品系成虫肠道微生物的影响

发布时间：2019-06-03 05:42

【摘要】：为研究辐照对瓜实蝇肠道微生物群落多样性和结构组成的影响,以100 Gy137Cs辐照来自瓜实蝇遗传区性品系(GSS)的5、8、11和13日龄的雌、雄成虫,并采用Illumina MiSeq测序技术分别对其肠道菌群进行测序分析。结果表明,瓜实蝇雌雄成虫的肠道中存在19个主要的菌属,包括17个属名已知,1个属名未知和1个属不可培养。其中,17个已知属分别属于4个细菌门,即变形菌门(Proteobacteria)(占总量的50%~98%)、厚壁菌门(Firmicutes)(占总量的2%~47%)、拟杆菌门(Bacteroidetes)(占总量的1%~16%)和1个至今微生物界还未确定的稀少菌门(unclassified rare phyla)(总量1%)。此外,辐照降低了瓜实蝇肠道中的微生物多样性以及优势菌的丰富度,且这种影响与瓜实蝇的性别和成虫日龄无关。本研究结果为评估和修复辐照对瓜实蝇的损伤提供了参考。
[Abstract]:In order to study the effect of irradiation on the diversity and structure of intestinal microbial community of Drosophila melanogaster, the male and female adults of 5, 8, 11 and 13 days old from the genetic strain (GSS) of Drosophila melanogaster were irradiated with 100 Gy137Cs. The intestinal flora was sequenced by Illumina MiSeq sequencing. The results showed that there were 19 main genus in the intestine of male and female adults of Drosophila melanogaster, including 17 genus names known, 1 genus name unknown and 1 genus unculturable. Among them, 17 known genus belong to 4 bacterial gates, that is, the (Proteobacteria) (of Proteus accounts for 50% 鈮，

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