不同地区青檀根际土壤肥力和真菌多样性及其相关性分析
发布时间：2019-06-09 20:51
【摘要】：为了探明不同地区青檀(Pteroceltis tatarinowii Maxim.)根际土壤真菌多样性及其与土壤肥力的关系,对国内7省(广西、安徽、河南、陕西、四川、山东和辽宁)1市(北京)8个样地野生青檀根际土壤的肥力指标(包括pH值、速效磷含量和速效钾含量)进行比较,并采用Illumina MiSeq高通量测序技术对其根际土壤的真菌多样性进行了分析;在此基础上,对土壤真菌多样性指数与土壤肥力指标的相关性以及土壤真菌科水平相对丰度前20位的操作分类单元(OTUs)与土壤肥力指标的相关性进行分析。结果表明:供试8个样地青檀根际土壤的pH值为pH 6.06~pH 7.61,速效磷含量为0.49~3.27μg·g~(-1),速效钾含量为150.24~636.89μg·g~(-1)。基于土壤真菌DNA测序结果,以有效序列相似度97%为阈值获得7 143个OTUs,鉴定出6门32纲106目254科,其中,包括子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和接合菌门(Zygomycota)3个优势门以及Incertae sedis(GenBank数据库中存在,但未准确分类的科)、被孢霉科(Mortierellaceae)、发菌科(Trichocomaceae)、丛赤壳科(Nectriaceae)和Archaeorhizomycetaceae 5个共有优势科;不同样地的土壤真菌多样性差异明显,Chao1指数、Shannon-Wiener指数和Simpson指数分别为1 041.37~1 793.19、5.39~6.14和0.007~0.020,盖度为98.90%~99.84%。相关性分析结果表明:土壤真菌Chao1指数与土壤速效钾含量呈显著(P0.05)负相关,其余土壤真菌多样性指数与土壤肥力指标的相关性均不显著;在相对丰度前20位的OTUs中,55%OTUs与土壤pH值呈负相关,并且,多数OTUs与土壤的速效磷含量和速效钾含量呈正相关,其中,OTU0(被孢霉科)与土壤pH值呈显著负相关,OTU1(丛赤壳科)和OTU4(伞型束梗孔菌科)(Agaricostilbaceae)与土壤速效钾含量呈显著正相关。研究结果显示:野生青檀根际土壤呈弱酸性至弱碱性,并具有富钾少磷的特点;青檀根际土壤真菌多样性与土壤肥力有一定相关性,并且,被孢霉科、丛赤壳科和伞型束梗孔菌科与土壤肥力有显著相关性。
[Abstract]:In order to find out the (Pteroceltis tatarinowii Maxim.) in different areas, The relationship between fungal diversity and soil fertility in rhizosphere soil was studied in 7 provinces (Guangxi, Anhui, Henan, Shaanxi, Sichuan, Shandong and Liaoning). The fertility indexes (including pH value) of wild green sandalwood rhizosphere soil in 8 sample plots (Beijing) were studied in 7 provinces of China (Guangxi, Anhui, Henan, Shaanxi, Sichuan, Shandong and Liaoning). The content of available phosphorus and available potassium were compared, and the fungal diversity in rhizosphere soil was analyzed by Illumina MiSeq high throughput sequencing technique. On this basis, the correlation between soil fungal diversity index and soil fertility index and the correlation between soil fertility index and operating classification unit (OTUs), which is the top 20 relative abundance of soil mycetes, were analyzed. The results showed that the pH value of Rhizosphere soil was pH 6.06~pH 7.61, the content of available phosphorus was 0.49 ~ 3.27 渭 g 路g ~ (- 1), and the content of available potassium was 150.24 ~ 636.89 渭 g 路g ~ (- 1). Based on the DNA sequencing results of soil fungi, 7143 OTUs, were obtained with 97% similarity of effective sequences to identify 6 phylum, 32 classes, 106 orders and 254 families, including ascomycetes (Ascomycota),. There are three dominant families of basidiomycetes, (Basidiomycota) and (Zygomycota), as well as Incertae sedis (GenBank database, but not accurately classified families). There are five dominant families, (Nectriaceae) and Archaeorhizomycetaceae, of (Mortierellaceae), (Trichocomaceae), and Archaeorhizomycetaceae, which are common to Basidiomycetes and Phyllostachys pubescens in the database of Basidiomycetes and Phyllostachys pubescens. There were significant differences in soil fungal diversity between different places. Chao1 index, Shannon-Wiener index and Simpson index were 1041.37 卤1 793.19, 5.39 卤6.14 and 0.007 卤0.020, respectively, and the coverage was 98.90% 99.84%. The results of correlation analysis showed that there was a significant negative correlation between soil fungal Chao1 index and soil available potassium content (P 0.05), but there was no significant correlation between soil fungal diversity index and soil fertility index. In OTUs with the top 20 relative abundance, 55%OTUs was negatively correlated with soil pH value, and most OTUs was positively correlated with available phosphorus content and available potassium content in soil, and OTU0 was negatively correlated with soil pH value. There was a significant positive correlation between OTU1 and OTU4 () (Agaricostilbaceae) and soil available potassium content. The results showed that the rhizosphere soil of wild sandalwood was weakly acidic to weakly alkaline, and had the characteristics of rich potassium and less phosphorus. There was a certain correlation between soil fungal diversity and soil fertility, and there was a significant correlation between soil fertility and Artemiaceae, Artemiaceae and Umbels.
【作者单位】： 安徽师范大学生命科学学院;
【基金】：国家自然科学基金资助项目(41401062) 中国科学院南京地质古生物研究所国家重点开放实验室开放基金项目(143115)
【分类号】：S714
本文编号：2495890
[Abstract]:In order to find out the (Pteroceltis tatarinowii Maxim.) in different areas, The relationship between fungal diversity and soil fertility in rhizosphere soil was studied in 7 provinces (Guangxi, Anhui, Henan, Shaanxi, Sichuan, Shandong and Liaoning). The fertility indexes (including pH value) of wild green sandalwood rhizosphere soil in 8 sample plots (Beijing) were studied in 7 provinces of China (Guangxi, Anhui, Henan, Shaanxi, Sichuan, Shandong and Liaoning). The content of available phosphorus and available potassium were compared, and the fungal diversity in rhizosphere soil was analyzed by Illumina MiSeq high throughput sequencing technique. On this basis, the correlation between soil fungal diversity index and soil fertility index and the correlation between soil fertility index and operating classification unit (OTUs), which is the top 20 relative abundance of soil mycetes, were analyzed. The results showed that the pH value of Rhizosphere soil was pH 6.06~pH 7.61, the content of available phosphorus was 0.49 ~ 3.27 渭 g 路g ~ (- 1), and the content of available potassium was 150.24 ~ 636.89 渭 g 路g ~ (- 1). Based on the DNA sequencing results of soil fungi, 7143 OTUs, were obtained with 97% similarity of effective sequences to identify 6 phylum, 32 classes, 106 orders and 254 families, including ascomycetes (Ascomycota),. There are three dominant families of basidiomycetes, (Basidiomycota) and (Zygomycota), as well as Incertae sedis (GenBank database, but not accurately classified families). There are five dominant families, (Nectriaceae) and Archaeorhizomycetaceae, of (Mortierellaceae), (Trichocomaceae), and Archaeorhizomycetaceae, which are common to Basidiomycetes and Phyllostachys pubescens in the database of Basidiomycetes and Phyllostachys pubescens. There were significant differences in soil fungal diversity between different places. Chao1 index, Shannon-Wiener index and Simpson index were 1041.37 卤1 793.19, 5.39 卤6.14 and 0.007 卤0.020, respectively, and the coverage was 98.90% 99.84%. The results of correlation analysis showed that there was a significant negative correlation between soil fungal Chao1 index and soil available potassium content (P 0.05), but there was no significant correlation between soil fungal diversity index and soil fertility index. In OTUs with the top 20 relative abundance, 55%OTUs was negatively correlated with soil pH value, and most OTUs was positively correlated with available phosphorus content and available potassium content in soil, and OTU0 was negatively correlated with soil pH value. There was a significant positive correlation between OTU1 and OTU4 () (Agaricostilbaceae) and soil available potassium content. The results showed that the rhizosphere soil of wild sandalwood was weakly acidic to weakly alkaline, and had the characteristics of rich potassium and less phosphorus. There was a certain correlation between soil fungal diversity and soil fertility, and there was a significant correlation between soil fertility and Artemiaceae, Artemiaceae and Umbels.
【作者单位】： 安徽师范大学生命科学学院;
【基金】：国家自然科学基金资助项目(41401062) 中国科学院南京地质古生物研究所国家重点开放实验室开放基金项目(143115)
【分类号】：S714
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