玉米秸秆低温高效降解复合菌系GF-20的温度和pH适应性研究
发布时间：2019-07-05 06:40
【摘要】：【目的】研究不同条件下复合菌系的秸秆分解能力和酶活性,评价该复合菌系的功能稳定性及适应性,为进一步完善该复合菌系的筛选技术及其应用提供理论依据。【方法】以复合菌系GF-20为研究对象,在不同温度和pH值条件下连续继代培养,测定所得复合菌系的滤纸分解特性、玉米秸秆降解率、发酵液pH值、纤维素酶和半纤维素酶活性,以评价复合菌系的功能稳定性。【结果】在温度4~30℃、pH 6.0~9.0时,经过15代的继代培养,复合菌系GF-20在培养的108~140h可分解滤纸纤维素,玉米秸秆降解率保持在30%以上,发酵液pH值随发酵时间的延长趋近于中性,pH调节能力良好,纤维素酶活性为1.22~2.34 U/mL,半纤维素酶活性为12.82~14.23U/mL。【结论】复合菌系GF-20可在较大的温度和pH范围内保持较高的纤维素和半纤维素酶活性,能发挥秸秆降解作用,具有良好的开发潜力和应用前景。
[Abstract]:[objective] to study the straw decomposition ability and enzyme activity of the compound strain under different conditions, to evaluate the functional stability and adaptability of the compound strain, and to provide theoretical basis for further perfecting the screening technology and application of the compound strain. [methods] taking the compound strain GF-20 as the research object, the filter paper decomposition characteristics and the degradation rate of corn straw were determined under different temperature and pH conditions. The pH value, cellulase and hemicellulase activity of fermentation broth were used to evaluate the functional stability of the compound strain. [results] when the temperature was 4 鈮，
本文编号：2510317
[Abstract]:[objective] to study the straw decomposition ability and enzyme activity of the compound strain under different conditions, to evaluate the functional stability and adaptability of the compound strain, and to provide theoretical basis for further perfecting the screening technology and application of the compound strain. [methods] taking the compound strain GF-20 as the research object, the filter paper decomposition characteristics and the degradation rate of corn straw were determined under different temperature and pH conditions. The pH value, cellulase and hemicellulase activity of fermentation broth were used to evaluate the functional stability of the compound strain. [results] when the temperature was 4 鈮，
本文编号：2510317
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