蜡蚧轮枝菌蛋白激发子对菜豆抗菜豆修尾蚜的研究

发布时间：2021-01-27 17:30

　　修尾蚜是一种危害广泛的农作物害虫,昆虫病原真菌蜡蚧轮枝菌（V.lecanii）和球孢白僵菌（B.bassiana）是一种对环境友好、靶标特异、能够有效防治多种刺吸式昆虫的生物农药。本研究测定了两株球孢白僵菌（B76和B252）和两株蜡蚧轮枝菌（L2和L5）对豆蚜的防治作用。用B76、B252、L2和L5及其二元组合（L2+B76、L2+B525、B76+L5和L2+B76+B252+L5）的滤液和分生孢子制剂进行了三次生物测定。在滤液和分生孢子的比较生物测定中,每个菌株的真菌滤液和分生孢子均为2ml。采用三种不同浓度(即1×10⁶、1×10⁷和1×10⁸ conidia mL^-1)进行二元组合生物测定。结果表明,在所有处理的第8天,对供试蚜虫的致病力最大。在孢子最高浓度为1×10⁸ conidia mL^-1的处理下,B76菌株（85.3%）和L2菌株（77.9%）的蚜虫死亡率均高于B252（66.0%）和L5（60.9%）。同样,B76和L2菌株的二元组合... 

【文章来源】：中国农业科学院北京市

【文章页数】：125 页

【学位级别】：博士

【文章目录】：
附件
摘要
ABSTRACT
Abbreviation
CHAPTER Ⅰ INTRODUCTION AND REVIEW OF LITERATURE
    1.1 APHIDS
        1.1.1 Control aphids by chemical pesticides
        1.1.2 Control aphids by biological pesticides
    1.2 INTERACTION OF APHID AND PLANT
    1.3 BIOLOGICAL CONTROL
        1.3.1 Type of biological control
        1.3.2 Type of biological control agents
        1.3.3 Advantages and disadvantages of biological control
    1.4 POTENTIAL OF ENTOMOPATHOGENIC FUNGI
        1.4.1 Entomopathogenic potential of Verticillium lecanii
        1.4.2 Entomopathogenic potential of Beauveria bassiana
    1.5 FUNGAL PROTEINS OR ENZYMES
        1.5.1 Chitinases
        1.5.2 Lipases
    1.6 PLANT DEFENCE AGAINST INSECT
    1.7 DEFENCE ELICITORS OF PLANT
    1.8 BIOTIC AND ABIOTIC RELATED STRESSES PATHWAY
        1.8.1 Salicylic acid signaling pathway
        1.8.2 Jasmonic acid signaling pathway
    1.9 OBJECTIVES
CHAPTER Ⅱ BIOCONTROL POTENTIAL OF SOME ENTOMOPATHOGENIC FUNGAL STRAINS AGAINST BEAN APHID MEGOURA JAPONICA MATSUMURA
    2.1 INTRODUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Insect culture
        2.2.2 Fungal isolates
        2.2.3 Conidial suspension
        2.2.4 Fungal filtrate
        2.2.5 Pathogenicity bioassays
        2.2.6 Data analysis
    2.3 RESULTS
        2.3.1 Inhibition effect of different concentrations of the fungal spore on bean aphid
        2.3.2 Fungal strain combinations against bean aphid
        2.3.3 Comparison of the mortality of bean aphid by fungi filtrate and conidia
CHAPTER Ⅲ IDENTIFICATION,PURIFICATION AND CHARACTERIZATION OF HR INDUCING PROTEIN IN TOBACCO
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Fungal strains
        3.2.2 Protein elicitor purification
        3.2.3 Gene identification
        3.2.4 Gene amplification and cloning
        3.2.5 Expression and purification of the recombinant protein elicitor
        3.2.6 Characterization of the Pev L1 protein elicitor
    3.3 RESULTS
        3.3.1 Purification of protein elicitor
        3.3.2 Protein Pev L1 induced hypersensitive response HR in tobacco leaves
        3.3.3 Gene amplification and cloning
        3.3.4 Expression and purification of recombinant protein elicitor Pev L1
CHAPTER Ⅳ A NOVEL PROTEIN ELICITOR PEVL1,FROM VERTICILLIUM LECANII STRAIN L2,INDUCES SYSTEMIC RESISTANCE AGAINST BEAN APHID（MEGOURA JAPONICA MATSUMURA）IN PHASEOLUS VULGARIS L
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Plant cultures and insect rearing
        4.2.2 Aphid choice test
        4.2.3 Effects of the Pev L1 protein on the reproductive biology of bean aphid
        4.2.4 Bioassays with Elicitor Protein Pev L1 for checking gene expression
        4.2.5 Plant RNA isolation and c DNA synthesis
        4.2.6 Reverse transcription-quantitative PCR（RT-q PCR）
        4.2.7 Experiment design and data analyses
    4.3 RESULTS
        4.3.1 Aphid choice test
        4.3.2 Effects of the Pev L1 protein on the reproductive biology of bean aphid
        4.3.3 Expression levels of SA-pathway genes in P.vulgaris L.
        4.3.4 Expression levels of JA-pathway genes in P.vulgaris L.
CHAPTER Ⅴ DISCUSSION AND SUMMARY
    5.1 DISCUSSION
    5.2 SUMMARY
CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
RESUME



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