Effects of Co-inoculating Soil Yeast （Saccharomyces Cerevisi

发布时间：2021-05-08 10:41

　　土壤中植物大量营养素（氮、磷、钾）的有效性是影响作物产量的主要因素之一。这些矿物元素的有效性和形式因土壤类型的不同而有很大的差异。研究表明,通过为植物提供适当的微生物共生体,可以优化土壤营养对植物的有效补充。此外,土壤酵母能够溶解不可利用的磷和铁,并产生植物生长激素,这些激素可帮助植物生长和维持细菌活性。本研究旨在探讨共接种植物促生长细菌（PGPB）—慢生大豆根瘤菌（Bradyrhizobium japonicum）和植物促生长真菌—土壤酵母（Saccharomyces cerevisiae和Saccharomyces exiguus）对大豆（Glycine maxL.Merr）生长和产量的影响,特别是土壤酵母如何影响细菌的空气氮固定率和结瘤率。本研究以台湾292大豆品种为试验对象,采用完全随机实验设计:接种慢生大豆根瘤菌B.japonicum(T1;接种土壤酵母S.cerevisiae(T2;接种S.exiguus（T3）;共接种土壤酵母S.cerevisiae和B.japonicum（T4）;共接种S.exiguous及B.japonicum（T5）以及对照（T6）,每个处理重复4... 

【文章来源】：西南科技大学四川省

【文章页数】：50 页

【学位级别】：硕士

【文章目录】：
摘要
ABSTRACT
Chapter 1
    1 Background
        1.1 Soybean production and demand
        1.2 Fertilization problems in soybean production
        1.3 Importance of microbes in crop production
        1.4 Research advancements in bio-fertilizer use in crop production
        1.5 Aims and objectives of the study
Chapter 2
    2 Materials and methods
        2.1 Plant material
        2.2 Microbial strains used
        2.3 Experimental design
        2.4 Experimental site location
        2.5 Green house experiment
        2.6 Soil characterisation
            2.6.1 Soil physiochemical parameters
            2.6.2 Soil p H determination
            2.6.3 Soil elemental analysis
        2.7 Inoculum preparation and seed treatment
        2.8 Data collection
            2.8.1 Plant sampling
                2.8.1.1 Soybean germination test
                2.8.1.2 Root elongation bioassay
                2.8.1.3 Nodulation
                2.8.1.4 Soil microbiological analysis
                2.8.1.5 Shoots for collection of plant sap (root bleeding) for determination of atmospheric nitrogen (N2) fixation, dry matter and determination of nitrogen (N) and phosphorus (P) in soybean leaves
                2.8.1.6 Determination of chlorophyll content
                2.8.1.7 Seeds for determination of the nutritional value of harvested seed
                2.8.1.8 Grain yield
                2.8.1.9 Correlation analysis among traits
        2.9 Data analysis
CHAPTER 3
    3 Results
        3.1 Soybean initial growth and development
        3.2 Soybean nodulation
        3.3 Atmospheric nitrogen fixation
        3.4 Yield parameters
Chapter 4
    4 Discussion
        4.1 Soybean initial growth and development
            4.1.1 Seedling shoot analysis
        4.2 Soybean nodulation analysis
            4.2.1 Nodule number and nodule dry weight
        4.3 Chlorophyll content (SPAD Values) leaf area and percentage of total plantnitrogen derived from atmospheric fixation (%Ndfa)
        4.4 Microbial count
        4.5 Yield parameters
Chapter 5
    5 Conclusion
Chapter 6
    6 Recommendations
Chapter 7
    7 Acknowledgements
List of References



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