甲氨基阿维菌素苯甲酸盐和氯虫苯甲酰胺对褐飞虱重要捕食性天敌青翅蚁型隐翅甲的风险评价
发布时间：2021-07-18 11:28
水稻持续增产是世界人口增长的必然要求。为了减少由于害虫爆发造成的作物损失,越来越多的不同新型杀虫剂被开发和应用。然而,杀虫剂对人类健康、环境和害虫自然天敌的危害同时也引起了公众的关注。天敌与害虫一般发生在同一个栖息地,这使得在利用杀虫剂控制害虫时天敌很容易通过任何直接途径或间接途径接触到杀虫剂。之前许多的研究也表明,杀虫剂对天敌的生长发育有重大影响。因此,杀虫剂对非目标生物的潜在不利影响评价是杀虫剂生物安全性评估不可缺少的一部分。青翅蚁型隐翅甲是一种重要的天敌种类,在世界范围内（中国、马来西亚、泰国和日本等地）被用于生物防治,可防治包括各种水稻害虫在内的多种毁灭性害虫。在水稻农业生态系统中,青翅蚁型隐翅甲是褐飞虱的重要天敌,其成虫每天可以捕食9-10只褐飞虱若虫。甲氨基阿维菌素苯甲酸盐和氯虫苯甲酰胺是防治不同作物上多种鞘翅目、鳞翅目和双翅目害虫的两种新型杀虫剂。在稻田中,这两种杀虫剂被大量的应用于防治稻纵卷叶螟和二化螟等水稻害虫中,但目前缺少这两种杀虫剂对青翅蚁型隐翅甲的安全性报道。本研究评价了甲氨基阿维菌素苯甲酸盐和氯虫苯甲酰胺对青翅蚁型隐翅甲的急性毒性,同时测定了亚致死剂量下两种杀...
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：139 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATION
CHAPTER Ⅰ INTRODUCTION
1.1 Food security and role of pesticide in food security
1.2 History and development of pesticides
1.3 Biological control
1.3.1 Classical biological control
1.3.2 Augmentative biological control
1.5 Rove beetle, Paederus fuscipes as biological control agent
1.6 Emamectin benzoate
1.6.1 History of development
1.6.2 Target insect
1.6.3 Chemistry
1.6.4 Mode of action
1.7 Chlorantraniliprole
1.7.1 History of development
1.7.2 Target insects
1.7.3 Chemistry
1.7.4 Mode of action
1.8 Insecticide risk assessment
1.8.1 Risk assessment of emamectin benzoate and chlorantraniliprole
1.9 Methods of risk assessment
1.9.1 Acute toxicity assessment
1.9.2 Chronic toxicity assessment
1.9.3 Biochemical assessment of toxicity
1.9.4 Transcriptome-based toxicity assessment
1.10 Objectives of research
CHAPTER Ⅱ Materials and methods
2.1 Insect culture rearing
2.1.1 Rearing of brown planthopper as host
2.1.2 Rearing of Paederus fuscipes as a predator
2.2 Insecticides and dilutions
2.3 Acute toxicity bioassay
2.4 Chronic toxicity bioassay
2.4.1 Developmental period assessment
2.4.2 Fecundity assessment bioassay
2.4.3 Feeding potential assessment bioassay
2.4.4 Body weight assessment
2.5 Biochemical induction bioassays
2.5.1 Exposure to Emamectin benzoate
2.5.2 Cytochrome P450 (P450) activity assessment
2.5.3 Glutathione S-transferase (GST) activity assessment
2.5.4 Carboxylesterase (CarEs) activity assessment
2.6 Transcriptome-based analysis under emamectin benzoate stress
2.6.1 Exposure to emamectin benzoate
2.6.2 Extraction, quantification and qualification of RNA
2.6.3 Library construction for transcriptome sequencing
2.6.4 Transcriptome data analysis
2.6.5 Gene analysis for differential expression
2.6.6 qRT-PCR for the validation of transcriptome analysis
2.7 Statistical Analysis
CHAPTER Ⅲ RESULTS
3.1 Short-term effects of Emamectin benzoate on P.fuscipes
3.1.1 Acute toxicity of Emamectin benzoate for adults and second instar larvae of P.fuscipes
3.2 Sublethal effects of insecticides on biological parameters of P. fuscipes
3.2.1 Sublethal effects of emamectin benzoate on developmental period of P. fuscipes
3.2.2 Sublethal effects of emamectin benzoate on fecundity of directly treated adults (10-days-old) of P.fuscipes
3.2.3 Sublethal effects of emamectin benzoate on fecundity of adults emerged from treated second instar larvae of P. fuscipes
3.2.4 Sublethal effects of emamectin benzoate on feeding potential of adults of P.fuscipes
3.2.5 Sublethal effects of emamectin benzoate on feeding potential of second instar larvae of P.fuscipes
3.2.6 Sublethal effects of emamectin benzoate on body weight of adult female of P.fuscipes
3.2.7 Sublethal effects of insecticides on body weight of adult male of P.fuscipes
3.3 The effects of chlorantraniliprole on P. fuscipes
3.3.1 Acute toxicity of chlorantraniliprole for adults and second instar larvae of P.fuscipes
3.4 Sublethal effects of insecticides on biological parameters of P.fuscipes
3.4.1 Sublethal effects of chlorantraniliprole on developmental period of P. fuscipes
3.4.2 Sublethal effects of insecticides on fecundity of directly treated adults (10-days-old) of P. fuscipes
3.4.3 Sublethal effects of insecticides on fecundity of adults emerged from treated second instar larvae of P. fuscipes
3.4.5 Sublethal effects of insecticides on feeding potential of adults of P. fuscipes
3.4.6 Sublethal effects of insecticides on feeding potential of second instar larvae of P.fuscipes
3.4.7 Sublethal effects of insecticides on body weight of adult female of P.fuscipes
3.4.8 Sublethal effects of insecticides on body weight of adult male of P. fuscipes
3.5 Effect of emamectin benzoate on detoxification enzymes activity
3.6 Identification of genes involved in detoxification mechanism of emamectin benzoate in P.fuscipes
3.6.1 Illumina sequencing and transcriptome analysis
3.6.2 Differential genes expression under emamectin benzoate stress
3.6.3 Detoxification mechanism related genes
3.6.4 Validation of transcriptome via qPCR
CHAPTER Ⅳ DISCUSSION
4.1 Lethal and sublethal effects of emamectin benzoate on P.fuscipes
4.2 Lethal and sublethal effects of chlorantraniliprole on P.fuscipes
4.3 Effect of emamectin benzoate on detoxification enzymes
4.4 Genetic variation due to emamectin benzoate exposure and gene regulation
CHAPTER V CONCLUSION AND FUTURE PERSPECTIVES
REFERENCE
SUPPLEMENT
PUBLICATIONS
ACKNOWLEDGEMENTS
【参考文献】：
期刊论文
[1]死浓度氯虫苯甲酰胺可降低亚洲玉米螟的种群增长(英文)[J]. 宋月芹,董钧锋,孙会忠. 昆虫学报. 2013(04)
[2]Sublethal Effects of Metaflumizone on Plutella xylostella （Lepidoptera: Plutellidae）[J]. ZHANG Zhe1, LI Jian-hong1 and GAO Xi-wu2 1 College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China 2 Department of Entomology, China Agricultural University, Beijing 100193, P.R.China. Journal of Integrative Agriculture. 2012(07)
[3]氯虫苯甲酰胺对黑肩绿盲蝽捕食功能的影响[J]. 王召,杨洪,金道超. 昆虫学报. 2012(05)
[4]重庆旱灾致一起隐翅虫皮炎暴发调查[J]. 蒋雪飞,李琴,廖云贞,陈亮. 现代预防医学. 2008(04)
本文编号：3289487
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：139 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATION
CHAPTER Ⅰ INTRODUCTION
1.1 Food security and role of pesticide in food security
1.2 History and development of pesticides
1.3 Biological control
1.3.1 Classical biological control
1.3.2 Augmentative biological control
1.5 Rove beetle, Paederus fuscipes as biological control agent
1.6 Emamectin benzoate
1.6.1 History of development
1.6.2 Target insect
1.6.3 Chemistry
1.6.4 Mode of action
1.7 Chlorantraniliprole
1.7.1 History of development
1.7.2 Target insects
1.7.3 Chemistry
1.7.4 Mode of action
1.8 Insecticide risk assessment
1.8.1 Risk assessment of emamectin benzoate and chlorantraniliprole
1.9 Methods of risk assessment
1.9.1 Acute toxicity assessment
1.9.2 Chronic toxicity assessment
1.9.3 Biochemical assessment of toxicity
1.9.4 Transcriptome-based toxicity assessment
1.10 Objectives of research
CHAPTER Ⅱ Materials and methods
2.1 Insect culture rearing
2.1.1 Rearing of brown planthopper as host
2.1.2 Rearing of Paederus fuscipes as a predator
2.2 Insecticides and dilutions
2.3 Acute toxicity bioassay
2.4 Chronic toxicity bioassay
2.4.1 Developmental period assessment
2.4.2 Fecundity assessment bioassay
2.4.3 Feeding potential assessment bioassay
2.4.4 Body weight assessment
2.5 Biochemical induction bioassays
2.5.1 Exposure to Emamectin benzoate
2.5.2 Cytochrome P450 (P450) activity assessment
2.5.3 Glutathione S-transferase (GST) activity assessment
2.5.4 Carboxylesterase (CarEs) activity assessment
2.6 Transcriptome-based analysis under emamectin benzoate stress
2.6.1 Exposure to emamectin benzoate
2.6.2 Extraction, quantification and qualification of RNA
2.6.3 Library construction for transcriptome sequencing
2.6.4 Transcriptome data analysis
2.6.5 Gene analysis for differential expression
2.6.6 qRT-PCR for the validation of transcriptome analysis
2.7 Statistical Analysis
CHAPTER Ⅲ RESULTS
3.1 Short-term effects of Emamectin benzoate on P.fuscipes
3.1.1 Acute toxicity of Emamectin benzoate for adults and second instar larvae of P.fuscipes
3.2 Sublethal effects of insecticides on biological parameters of P. fuscipes
3.2.1 Sublethal effects of emamectin benzoate on developmental period of P. fuscipes
3.2.2 Sublethal effects of emamectin benzoate on fecundity of directly treated adults (10-days-old) of P.fuscipes
3.2.3 Sublethal effects of emamectin benzoate on fecundity of adults emerged from treated second instar larvae of P. fuscipes
3.2.4 Sublethal effects of emamectin benzoate on feeding potential of adults of P.fuscipes
3.2.5 Sublethal effects of emamectin benzoate on feeding potential of second instar larvae of P.fuscipes
3.2.6 Sublethal effects of emamectin benzoate on body weight of adult female of P.fuscipes
3.2.7 Sublethal effects of insecticides on body weight of adult male of P.fuscipes
3.3 The effects of chlorantraniliprole on P. fuscipes
3.3.1 Acute toxicity of chlorantraniliprole for adults and second instar larvae of P.fuscipes
3.4 Sublethal effects of insecticides on biological parameters of P.fuscipes
3.4.1 Sublethal effects of chlorantraniliprole on developmental period of P. fuscipes
3.4.2 Sublethal effects of insecticides on fecundity of directly treated adults (10-days-old) of P. fuscipes
3.4.3 Sublethal effects of insecticides on fecundity of adults emerged from treated second instar larvae of P. fuscipes
3.4.5 Sublethal effects of insecticides on feeding potential of adults of P. fuscipes
3.4.6 Sublethal effects of insecticides on feeding potential of second instar larvae of P.fuscipes
3.4.7 Sublethal effects of insecticides on body weight of adult female of P.fuscipes
3.4.8 Sublethal effects of insecticides on body weight of adult male of P. fuscipes
3.5 Effect of emamectin benzoate on detoxification enzymes activity
3.6 Identification of genes involved in detoxification mechanism of emamectin benzoate in P.fuscipes
3.6.1 Illumina sequencing and transcriptome analysis
3.6.2 Differential genes expression under emamectin benzoate stress
3.6.3 Detoxification mechanism related genes
3.6.4 Validation of transcriptome via qPCR
CHAPTER Ⅳ DISCUSSION
4.1 Lethal and sublethal effects of emamectin benzoate on P.fuscipes
4.2 Lethal and sublethal effects of chlorantraniliprole on P.fuscipes
4.3 Effect of emamectin benzoate on detoxification enzymes
4.4 Genetic variation due to emamectin benzoate exposure and gene regulation
CHAPTER V CONCLUSION AND FUTURE PERSPECTIVES
REFERENCE
SUPPLEMENT
PUBLICATIONS
ACKNOWLEDGEMENTS
【参考文献】：
期刊论文
[1]死浓度氯虫苯甲酰胺可降低亚洲玉米螟的种群增长(英文)[J]. 宋月芹,董钧锋,孙会忠. 昆虫学报. 2013(04)
[2]Sublethal Effects of Metaflumizone on Plutella xylostella （Lepidoptera: Plutellidae）[J]. ZHANG Zhe1, LI Jian-hong1 and GAO Xi-wu2 1 College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China 2 Department of Entomology, China Agricultural University, Beijing 100193, P.R.China. Journal of Integrative Agriculture. 2012(07)
[3]氯虫苯甲酰胺对黑肩绿盲蝽捕食功能的影响[J]. 王召,杨洪,金道超. 昆虫学报. 2012(05)
[4]重庆旱灾致一起隐翅虫皮炎暴发调查[J]. 蒋雪飞,李琴,廖云贞,陈亮. 现代预防医学. 2008(04)
本文编号：3289487
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