油茶树根际土壤解有机磷细菌的分离、鉴定及解磷能力分析（英文）

发布时间：2023-04-11 03:17

　　为了获得高效解有机磷细菌,从油茶植株根际土壤中分离到21株解有机磷细菌,采用透明圈法进行复筛,仅有4株细菌能在有机磷培养基上形成明显的透明圈。4株细菌的D/d值在1.62～2.71之间,其中菌株Y6的D/d值最大,为2.71。发酵上清液有效磷含量为8.50～14.79 mg/L,较CK增加7.88～14.17 mg/L,其中菌株Y6发酵上清液有效磷含量最多,为14.79 mg/L,较CK增加14.17 mg/L。根据菌株Y6的菌落形态特征和生理生化特征及16S rDNA序列分析结果,初步确定菌株Y6为假单胞菌属。该菌株有利于改善油茶磷素供应,促进油茶生长,在生物有机肥研制中具有较大潜力。

【文章页数】：7 页

【文章目录】：
1. Introduction
2. Materials and Methods
    2.1. Test material
        2.1.1. Sample collection
        2.1.2. Culture medium
    2.2. Test method
        2.2.1. Isolation and purification of organophosphorus solubilizing bacteria
        2.2.2. Rescreening of organophosphorus solubilizing bacteria
        2.2.3. Analysis of phosphorus solubilizing ability
        2.2.4. Identification of organophosphorus solubilizing bacteria
        2.2.5. The relationship between the content of available phosphorus and pH value in the fermentation broth of organophosphorus solubilizing bacteria
        2.2.6. Determination of the growth curve of organophosphorus solubilizing bacteria
        2.2.7. Data processing
3. Results and Analysis
    3.1. Isolation and purification of organoph-osphorus solubilizing bacteria
    3.2. Rescreening of organophosphorus solubilizing bacteria
    3.3. Analysis of the ability of organophosphorus solubilizing bacteria to solubilize phosphorus
    3.4. Identification of organophosphorus solub-ilizing bacteria
        3.4.1. Morphological identification of strains
        3.4.2. Physiological and biochemical characteristics of strains
        3.4.3.16S rDNA sequence analysis of the strain
    3.5. The relationship between pH value and available phosphorus content of fermentation liquid of strain Y6
    3.6. Growth curve of organophosphorus solub-ilizing bacteria Y6
4. Conclusion and Discussion



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