昆虫病原真菌球孢白僵菌ARSEF2860及其分离的蛋白激发子PeBA1抗烟粉虱的活性分析
发布时间：2024-05-18 02:38
1.烟粉虱是田间作物和温室中最具破坏性的害虫之一。本研究旨在4个不同温度条件下(20、24、28和32°C),通过表面喷洒的方式评估2种白僵菌菌株(BB-72和BB-252)和1种蜡蚧轮枝菌(V-2)对烟粉虱的体外杀虫活性。使用三种不同的处理进行生物测定,分别包括真菌菌株BB-72,BB-252和V-2的分生孢子浓度和滤液,以及它们的二元组合。处理一:将取每种真菌菌株的1.5 mL培养滤液进行生物测定;处理二:使用每种菌株的不同浓度的分生孢子悬浮液(1×106 mL-1、1×107mL-1和1×108 mL-1)进行生测,处理三:1 mL分生孢子和1 mL滤液的二元组合,例如BB-72×BB-72(1:1),BB-252×BB-252(1:1)和V2×V2(1:1)。结果显示在处理后的第12天统计烟粉虱的累计死亡率。在24°C条件下使用最高浓度(1×108mL-1)分生孢子处理,烟粉虱死亡率最高,BB-72处理死...
【文章页数】：107 页
【学位级别】：博士
【文章目录】：
博士学位论文评阅人、答辩委员会签名表
摘要
abstract
Chapter1 Introduction
1.1 Tomato
1.2 Whitefly
1.3 Whitefly and tomato
1.4 Whitefly feeding behaviour
1.5 Nature of damage of Whiteflies
1.6 Virus transmission
1.7 Whitefly control
1.8 Entomopathogenic Fungi
1.9 How Do Entomopathogenic Fungi(EPFs)Behave?
1.10 Beauveria bassiana
1.10.1 Morphology,cultural characteristics and molecular characterization of Beauveria bassiana
1.10.2 Life cycle of Beauveria bassiana
1.10.3 Factors responsible for germination of conidia of Beauveria bassiana
1.10.4 Pathogenicity of Beauveria bassiana
1.10.5 Beauveria bassiana as a Biocontrol agent
1.10.6 Agricultural importance of Beauveria bassiana
1.11 Verticillium lecanii (Lecanicillium lecanii)
1.11.1 Insecticidal potential of V.lecanii
1.12 Defense Mechanisms of Plants to Insect Pests
1.13 Host Plant Defenses against Insects
1.14 Morphological features for physical defense
1.15 Elicitors
1.16 Use of elicitors in defense mechanism
1.17 Classification of elicitors
1.17.1 General elicitors
1.17.2 Specific elicitors(Effectors)
1.18 Secondary metabolites for chemical defense of plants
1.19 Plant defensive proteins
1.20 Herbivore induced plant volatiles(HIPVs)
1.21 Defense elicitors(insect oral secretion)
1.22 Role of phytohormones in induced resistance in plants
Chapter2 In Vitro Pathogenicity of the fungi;Beauveria bassiana and Lecanicillium lecanii at different temperatures against the Whitefly,Bemisia tabaci(Genn.) (Hemiptera:Aleyrodidae)
2.1 Introduction
2.2 Materials and Methods
2.2.1 Whitefly stock culture
2.2.3 Effect of temperature on fungus virulence against Bemisia tabaci
2.2.4 Fungal isolates
2.2.5 Conidial suspensions
2.2.6 Fungal filtrate
2.2.7 Pathogenicity Bioassays
2.2.8 Data Analysis
2.3 Results
2.3.1 Conidial Virulence
2.3.2 Filtrate Pathogenicity
2.3.3 Binary Combinations Entomopathogenicity
2.4 Discussion
Chapter3 Effect of partially purified protein derived from Beauveria bassiana on expression of defense related genes in tomato against whitefly
3.1 Introduction
3.2 Material Method
3.2.1 Plant culture and insect rearing
3.2.2 Fungal culture
3.2.3 Crude protein preparation and partial purification
3.2.4 Protein activity bioassay against B.tabaci
3.2.5 RNA extraction and c DNA synthesis
3.2.6 Reverse transcription-quantitative PCR(RT-q PCR)
3.2.7 Statistical analysis
3.3 Result
3.3.1 Purification of the Beauveria bassiana ARSEF2860 crude protein
3.3.2 B.bassiana protein effect on B.tabaci survival
3.3.3 Effect of B.bassiana derivative protein on fecundity B.tabaci
3.3.4 Plant defense gene expression level in response to B.bassiana protein
3.4 Discussion
3.5 Conclusions
Chapter4 Protein elicitor Pe BA1enhanced resistance against whitefly Bemisia tabaci(Genn.) (Hemiptera:Aleyrodidae)in tomato(Solanum lycopersicum)
4.1 Introduction
4.2 Material methods
4.2.1 Insects and host plants
4.2.2 Protein purification
4.2.3 Characterization of protein
4.2.4 Isolation of RNA and c DNA synthesis:
4.2.5 Reverse Transcription–Quantitative PCR(RT-q PCR)
4.2.6 Statistical analysis
4.3 Results
4.3.1 Expression,purification and evaluation of Pe BA1 elicitor protein
4.3.2 Effect of Pe BA1 Elicitor on the Fecundity Rate
4.3.3 Expression of Plant Defense
Discussion
Conclusions
References
ACKNOWLEDGEMENTS
Resume
本文编号：3976331
【文章页数】：107 页
【学位级别】：博士
【文章目录】：
博士学位论文评阅人、答辩委员会签名表
摘要
abstract
Chapter1 Introduction
1.1 Tomato
1.2 Whitefly
1.3 Whitefly and tomato
1.4 Whitefly feeding behaviour
1.5 Nature of damage of Whiteflies
1.6 Virus transmission
1.7 Whitefly control
1.8 Entomopathogenic Fungi
1.9 How Do Entomopathogenic Fungi(EPFs)Behave?
1.10 Beauveria bassiana
1.10.1 Morphology,cultural characteristics and molecular characterization of Beauveria bassiana
1.10.2 Life cycle of Beauveria bassiana
1.10.3 Factors responsible for germination of conidia of Beauveria bassiana
1.10.4 Pathogenicity of Beauveria bassiana
1.10.5 Beauveria bassiana as a Biocontrol agent
1.10.6 Agricultural importance of Beauveria bassiana
1.11 Verticillium lecanii (Lecanicillium lecanii)
1.11.1 Insecticidal potential of V.lecanii
1.12 Defense Mechanisms of Plants to Insect Pests
1.13 Host Plant Defenses against Insects
1.14 Morphological features for physical defense
1.15 Elicitors
1.16 Use of elicitors in defense mechanism
1.17 Classification of elicitors
1.17.1 General elicitors
1.17.2 Specific elicitors(Effectors)
1.18 Secondary metabolites for chemical defense of plants
1.19 Plant defensive proteins
1.20 Herbivore induced plant volatiles(HIPVs)
1.21 Defense elicitors(insect oral secretion)
1.22 Role of phytohormones in induced resistance in plants
Chapter2 In Vitro Pathogenicity of the fungi;Beauveria bassiana and Lecanicillium lecanii at different temperatures against the Whitefly,Bemisia tabaci(Genn.) (Hemiptera:Aleyrodidae)
2.1 Introduction
2.2 Materials and Methods
2.2.1 Whitefly stock culture
2.2.3 Effect of temperature on fungus virulence against Bemisia tabaci
2.2.4 Fungal isolates
2.2.5 Conidial suspensions
2.2.6 Fungal filtrate
2.2.7 Pathogenicity Bioassays
2.2.8 Data Analysis
2.3 Results
2.3.1 Conidial Virulence
2.3.2 Filtrate Pathogenicity
2.3.3 Binary Combinations Entomopathogenicity
2.4 Discussion
Chapter3 Effect of partially purified protein derived from Beauveria bassiana on expression of defense related genes in tomato against whitefly
3.1 Introduction
3.2 Material Method
3.2.1 Plant culture and insect rearing
3.2.2 Fungal culture
3.2.3 Crude protein preparation and partial purification
3.2.4 Protein activity bioassay against B.tabaci
3.2.5 RNA extraction and c DNA synthesis
3.2.6 Reverse transcription-quantitative PCR(RT-q PCR)
3.2.7 Statistical analysis
3.3 Result
3.3.1 Purification of the Beauveria bassiana ARSEF2860 crude protein
3.3.2 B.bassiana protein effect on B.tabaci survival
3.3.3 Effect of B.bassiana derivative protein on fecundity B.tabaci
3.3.4 Plant defense gene expression level in response to B.bassiana protein
3.4 Discussion
3.5 Conclusions
Chapter4 Protein elicitor Pe BA1enhanced resistance against whitefly Bemisia tabaci(Genn.) (Hemiptera:Aleyrodidae)in tomato(Solanum lycopersicum)
4.1 Introduction
4.2 Material methods
4.2.1 Insects and host plants
4.2.2 Protein purification
4.2.3 Characterization of protein
4.2.4 Isolation of RNA and c DNA synthesis:
4.2.5 Reverse Transcription–Quantitative PCR(RT-q PCR)
4.2.6 Statistical analysis
4.3 Results
4.3.1 Expression,purification and evaluation of Pe BA1 elicitor protein
4.3.2 Effect of Pe BA1 Elicitor on the Fecundity Rate
4.3.3 Expression of Plant Defense
Discussion
Conclusions
References
ACKNOWLEDGEMENTS
Resume
本文编号：3976331
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