3D生物打印的微结构促进小鼠表皮干细胞的增殖和活性

发布时间：2018-11-17 18:40

【摘要】：目的探索不同构架的3D微结构对表皮干细胞增殖能力和细胞活性的影响并建立最佳3D生物打印模型。方法通过采用不同尺寸:210、340、420μm的打印喷头结合3D生物打印技术构建3种不同的含细胞3D微结构;利用荧光显微镜观察3D微结构中细胞形态及增殖现象;活/死细胞染色技术检测细胞活性;采用方差分析和样本t检验等方法进行统计学分析。结果 3种不同构架的3D微结构均可促进表皮干细胞增殖;打印后0、3、7 d之间,3组3D微结构在细胞活性水平上均逐步降低且差异有统计学意义(P0.01);与7 d时的细胞活性相比,3组3D微结构在14 d时的细胞活性均升高且差异有统计学意义(P0.01);与210μm组和340μm组相比,420μm组3D微结构在长期培养中细胞活性水平最高(P0.01)。结论 420μm组3D微结构能够稳定促进皮肤替代物中表皮干细胞的增殖能力并维持高细胞活性,为构建3D生物打印组织工程表皮以及全层皮肤模型奠定了基础。
[Abstract]:Objective to explore the effects of 3D microstructures on the proliferation and cell activity of epidermal stem cells (ESCs) and to establish the best 3D bioprinting model. Methods three kinds of cell-containing 3D microstructures were constructed by using different sizes: 210340420 渭 m printing nozzle and 3D bio-printing technology, and the cell morphology and proliferation in 3D microstructures were observed by fluorescence microscope. Live / dead cell staining technique was used to detect cell activity and statistical analysis was carried out by means of variance analysis and t-test. Results 3D microstructures of three different structures could promote the proliferation of epidermal stem cells, and within 7 days after printing, the three groups of 3D microstructures gradually decreased in the level of cell activity and the differences were statistically significant (P0.01). Compared with 7 days, the cell activity of 3D microstructures in the three groups increased at 14 days and the difference was statistically significant (P0.01). Compared with 210 渭 m and 340 渭 m groups, 420 渭 m group had the highest level of cell activity in long-term culture (P0.01). Conclusion the 3D microstructures of 420 渭 m group can stably promote the proliferation of epidermal stem cells in skin substitutes and maintain high cell activity, which lays a foundation for the construction of 3D bioprinted tissue engineered epidermis and full-thickness skin model.
【作者单位】：南方医科大学;南方医科大学南方医院胸外科;解放军总医院基础医学研究所;解放军总医院第一附属医院全军创伤修复与组织再生重点实验室暨皮肤损伤修复与组织再生北京市重点实验室;南开大学医学院;
【分类号】：R318.08;TP391.73

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