结构光照明显微中的超分辨图像重建研究

发布时间：2019-03-16 13:23

【摘要】：近年来,随着各种新型荧光探针的出现和成像方法的改进,远场光学成像的分辨率已经突破了衍射极限的限制。基于结构光照明的荧光显微技术凭借成像速度快、光毒性弱等优点,已成为目前主流的超分辨成像技术之一。实现结构光照明超分辨显微成像的关键在于照明光场的精准调控和后期的超分辨图像重建算法,否则将会在重建的超分辨图像中产生不可预估的伪影,混淆对观测结构真实形态的判断。详细对比了几种典型的结构光照明显微超分辨重建算法,证明基于图像重组变换的结构光照明超分辨图像重建算法可以有效解决极低结构光场调制度下的超分辨图像重建问题,降低结构光照明显微中的激发光功率。
[Abstract]:In recent years, with the emergence of various new fluorescent probes and the improvement of imaging methods, the resolution of far-field optical imaging has broken through the limitation of diffraction limit. Fluorescence microscopy based on structured light illumination has become one of the most popular super-resolution imaging techniques because of its advantages such as fast imaging speed and low phototoxicity. The key to realize the structure illumination super-resolution microscopic imaging is the precise regulation of the lighting field and the later super-resolution image reconstruction algorithm. Otherwise, it will produce the unpredictable artifact in the reconstructed super-resolution image. Confusion of the judgment of the true shape of the observation structure. In this paper, several typical micro-super-resolution reconstruction algorithms of structural illumination are compared in detail, and it is proved that the super-resolution reconstruction algorithm based on image recombination transform can effectively solve the problem of super-resolution image reconstruction under very low light-field modulation. The excitation power in the obvious micro-illumination of the structure is reduced.
【作者单位】：中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室;
【基金】：国家自然科学基金(61522511,11404389,81427802,61275193) 陕西省自然科学基础研究计划(2016JZ020)
【分类号】：TP391.41

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