酵母菌油脂含量测定方法的比较研究

发布时间：2018-04-27 22:29

本文选题：酵母菌 + 油脂含量　；参考：《中国油脂》2016年11期

【摘要】：为确定一种快速简便、准确可靠的酵母菌油脂含量测定方法,对磷酸香草醛显色法、氧化酶法与重量法进行比较分析。结果表明:在测定酵母菌体总脂含量时重量法准确度和精确度高,成本低,但操作过程烦琐、耗时长,溶剂有毒易污染环境;磷酸香草醛显色法的平均回收率约为93%,准确度和精确度较高,耗时少,最低检测限约为0.08 g/L,测定时需与相应的菌体油脂标准曲线匹配使用,其测定发酵液油脂含量与重量法差异不显著(P0.05),可以作为酵母菌油脂含量测定的方法;在测定酵母菌体内甘油三酯含量时,氧化酶法平均回收率达到98.0%,准确度和精确度高,该方法操作简便,耗时少,最低检测限为1.2 g/L;氧化酶法需与甘油三酯标准曲线匹配使用,同时还需对细胞进行破壁,但由于其快速准确性,也不失为一种酵母菌油脂含量测定的好方法。
[Abstract]:In order to determine a rapid, simple, accurate and reliable method for the determination of yeast oil, the colorimetric method of vanillin phosphate, oxidase method and gravimetric method were compared and analyzed. The results showed that the gravimetric method had high accuracy and accuracy and low cost in the determination of total lipid content of yeast, but the operation process was cumbersome, the time was long, and the solvent was toxic and easy to pollute the environment. The average recovery of vanillin phosphate colorimetric method is about 933, the accuracy and accuracy are higher, the time consuming is less, the minimum detection limit is about 0.08 g / L, and the determination needs to be matched with the corresponding bacterial oil standard curve. The determination of lipid content in fermentation broth was not significantly different from that by gravimetric method, which could be used as a method for the determination of lipid content of yeast, and the average recovery rate of oxidase method was 98.0 and the accuracy and accuracy were high when determining the content of triglyceride in yeast. The method is simple and time-consuming, with a minimum detection limit of 1.2 g / L. Oxidase method is used to match the standard curve of triglyceride and to break the cell wall, but because of its rapid accuracy, It is also a good method for the determination of the oil content of Saccharomyces cerevisiae.
【作者单位】：海南大学食品学院;南阳理工学院生物与化学工程学院;南阳市工业微生物重点实验室;车用生物燃料技术国家重点实验室;
【基金】：河南省基础与前沿技术研究项目(132300410087)
【分类号】：Q936;TE667

【相似文献】