漆酶的固定化及应用于循环水系统除油的研究

发布时间：2018-07-02 13:39

本文选题：漆酶 + 固定化　；参考：《中国石油大学(华东)》2015年硕士论文

【摘要】：在我国石油炼化企业中,普遍存在着油品泄漏问题。油品泄漏导致循环水中含有大量石油烃类物质,从而影响生产装置的正常运行。生物酶是一种绿色环保生物催化剂,可以有效的降解油类物质。针对游离漆酶易受环境条件影响变性失活且反应体系不容易分离回收等缺点,本文采用固定化漆酶替代游离酶,克服了游离酶的不足,并研究了漆酶的固定化以及在柴油泄漏的循环水中的除油效果。主要内容包括:(1)对包埋法、交联法和包埋-交联法这三种固定化酶的方法进行了研究,确定了固定化的最优条件;(2)优化了处理含柴油循环冷却水的工艺条件;(3)研究了常规缓蚀剂PASP与ATMP与固定化漆酶的协同作用;(4)考察了将固定化漆酶复合药剂与活性炭共同制成滤柱,在实际应用时的除油率。实验结果表明:采用交联法的固定化漆酶的除油效果最好,最大能降解77.2%的柴油,且固定化酶反复使用6次时,除油率仍能达到44%。将常规缓蚀剂中缓蚀效果较好的ATMP与漆酶共同固定在壳聚糖载体上,对固定化漆酶缓蚀性能进行优化,固定化漆酶的缓蚀率从16.22%增加到46.89%。固定化漆酶复合药剂与活性炭一起填充到滤柱中,泵流量为0.9L/h,反复通过滤柱5次除油率为92.8%,装有等量活性炭的滤柱除油率为30%,从而证明固定化漆酶滤柱除油的可行性。
[Abstract]:Oil leakage is a common problem in petroleum refining and chemical enterprises in China. Oil leakage leads to a large amount of petroleum hydrocarbons in circulating water, which affects the normal operation of the production plant. Bioenzyme is a kind of green biocatalyst, which can degrade oil effectively. In this paper, the immobilized laccase is used to replace the free laccase, which is easy to be deactivated and deactivated under the influence of environmental conditions, and the reaction system is not easy to separate and recover, which overcomes the deficiency of free laccase. The immobilization of laccase and the deoiling effect of laccase in diesel-leaking circulating water were studied. The main contents are as follows: (1) the three immobilized enzyme methods, including entrapment method, crosslinking method and entrapping crosslinking method, were studied. The optimum conditions of immobilization were determined; (2) the process conditions of treating the circulating cooling water containing diesel oil were optimized; (3) the synergistic action of conventional corrosion inhibitor PASP and ATMP with immobilized laccase was studied; (4) the combination of immobilized laccase and its activity was investigated. Carbon is used together to form filter columns, Oil removal rate in practical application. The results showed that the immobilized laccase with crosslinking method had the best deoiling effect, and the maximum degradation rate of diesel oil was 77.2%. When the immobilized laccase was used repeatedly for 6 times, the oil removal rate could still reach 44%. The inhibition rate of the immobilized laccase was increased from 16.22% to 46.89 by immobilization of ATMP and laccase on chitosan carrier. The immobilized laccase compound agent was filled into the filter column together with activated carbon, the pump flow rate was 0.9L / h, the oil removal rate was 92.8 through the filter column 5 times repeatedly, and the oil removal rate of the filter column containing the same amount of activated carbon was 30%, which proved the feasibility of the immobilized laccase filter column oil removal.
【学位授予单位】：中国石油大学(华东)
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：Q814.2;TE62

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