蛋白组学研究抗菌肽对BEL-7402细胞的作用

发布时间：2018-02-01 12:01

本文关键词： 抗菌肽cecropin BEL-细胞蛋白组学　出处：《生物技术》2010年06期 　论文类型：期刊论文

【摘要】：目的:采用蛋白组学研究家蝇抗菌肽cecropin对人肝癌BEL-7402细胞蛋白的影响,从蛋白质水平探讨抗菌肽抗肿瘤机制。方法:在测定肿瘤细胞生长指数的基础上,通过双向凝胶电泳分离差异蛋白,银染后进行图像分析,对表达差异2倍以上蛋白质点进行胶内酶解和MALDI-TOF-MS检测,获得肽质量指纹谱,应用Mascot搜索引擎在NCBI nr数据库中进行检索鉴定。结果:家蝇抗菌肽cecropin能够抑制BEL-7402细胞的生长,与对照组比较,抗菌肽处理组双向凝胶电泳共鉴定出12个差异表达蛋白点(上调蛋白6个,下调蛋白6个),大部分与细胞凋亡、细胞代谢、基因转录以及细胞骨架等相关。结论:这为深入研究抗菌肽抗肿瘤机制打下了基础。
[Abstract]:Objective: to study the effect of housefly antimicrobial peptide cecropin on human hepatoma BEL-7402 cell protein by proteomics. Methods: on the basis of measuring the growth index of tumor cells, differential proteins were separated by two-dimensional gel electrophoresis and analyzed by image after silver staining. The peptide mass fingerprint was obtained by endonuclease hydrolysis and MALDI-TOF-MS detection. Mascot search engine was used to search and identify NCBI nr database. Results: housefly antimicrobial peptide cecropin could inhibit the growth of BEL-7402 cells. Compared with the control group, 12 differentially expressed protein spots (6 up-regulated proteins and 6 down-regulated proteins) were identified by two-dimensional gel electrophoresis in the antimicrobial peptide treatment group, most of which were associated with cell apoptosis and cell metabolism. Conclusion: this provides a basis for further study on antitumor mechanism of antimicrobial peptides.
【作者单位】：广东药学院基础学院;广东省生物活性药物研究重点实验室;
【基金】：广东省科技攻关项目(“家蝇抗菌肽cecropin抗肿瘤作用及其机理的实验研究,”No.2010B031200011) 广州市科技攻关项目(“家蝇抗菌肽的制备及生物功能评价,”No.2005Z3-E0211)资助
【分类号】：R735.7
【正文快照】： 抗菌肽(antibacterical peptides ABPs)是存在于生物体内具有抵抗外界微生物侵害、消除体内突变细胞的一类小分子多肽,在动物免疫细胞、脏器、皮肤,植物及微生物中广泛存在[1]。研究发现,它不仅可以作用于细菌、真菌、某些寄生虫,而且能够抑制某些肿瘤细胞的增殖,但其抗肿瘤

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