蛇毒蛋白质组学研究进展

发布时间：2018-04-09 06:24

本文选题：蛇毒　切入点：蛋白质组学　出处：《陕西师范大学学报(自然科学版)》2008年06期

【摘要】：就蛇毒蛋白质组学研究的主要方法、所取得的成就及存在的问题进行了综述.利用高通量质谱技术对蛇毒组分进行全方位研究,是近年来蛋白组学研究中的一个热点.蛇毒蛋白质组学研究包括粗毒分离、质谱分析及生物信息学鉴定三个部分.粗毒分离的主要方法包括双向电泳、凝胶过滤及反相-高效液相色谱;质谱分析的方法包括基质辅助激光解离-飞行时间质谱、液相色谱质谱联用、电喷雾离子化串联质谱和电喷雾离子化傅立叶变换离子回旋共振质谱;而利用MS-Fit或Mascot搜索引擎访问相应的蛋白质专门网站对质谱所获肽序进行查询则是生物信息学鉴定的主要方法.迄今,利用蛋白质组技术已经对游蛇科、眼镜蛇科、蝰科中的58个属100多种(亚种)蛇毒的毒物进行了研究,鉴定出了十多个蛋白质家族.这些结果不仅有助于增强人们对毒素进化与分化以及中毒机理的了解,并对新药设计中的先导化合物寻找具有重要意义.未来,蛇毒蛋白质组学研究在与高通量蛇毒组的绝对定量及所鉴定蛋白的功能表征有关的方法技术方面仍有待突破.
[Abstract]:The main methods, achievements and problems in proteomics of snake venom were reviewed.The study of snake venom components by high throughput mass spectrometry (HMS) has been a hotspot in proteomics in recent years.The proteomics study of snake venom includes three parts: isolation of crude venom, mass spectrum analysis and bioinformatics identification.The main methods for the separation of crude toxins include two-dimensional electrophoresis, gel filtration and reversed-phase high performance liquid chromatography; mass spectrometry methods include matrix assisted laser dissociation-time-of-flight mass spectrometry, liquid chromatography-mass spectrometry,Electrospray ionization tandem mass spectrometry and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry;The main method of bioinformatics identification is to use MS-Fit or Mascot search engine to visit the corresponding protein website to query the peptide sequence obtained by mass spectrometry.Up to now, more than 10 families of proteins have been identified by proteome technique in 58 genera and 100 species of venom of Viperidae, Cobra and Viperidae.These results not only help to enhance the understanding of the evolution and differentiation of toxins and the mechanism of poisoning, but also play an important role in finding leading compounds in the design of new drugs.In the future, the proteomics of snake venom needs to be improved in terms of absolute quantification of high-throughput snake venom and functional characterization of identified proteins.
【作者单位】：陕西师范大学生命科学学院;西安回天血液制品有限责任公司;
【基金】：国家自然科学基金资助项目(30870303) 陕西省自然科学基金资助项目(SJ08C102)
【分类号】：R99

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