胰腺癌差异蛋白质组学研究
发布时间：2018-11-05 14:38
【摘要】：目的通过差异蛋白质组学研究,确定胰腺癌组织及相应正常胰腺组织之间蛋白质表达谱的差异,进而阐明其表达水平的变化与胰腺癌发生发展之间的关系。方法选取胰腺癌组织及相应正常胰腺组织6对,提取总蛋白进行双向聚丙烯酰胺凝胶电泳,经PDQuest软件分析,获得差异表达蛋白质点。差异蛋白质点经过切取、酶解后进行电喷雾四极杆飞行时间质谱(ESI-Q-TOF MS/MS)鉴定。质谱数据使用MASCOT搜索引擎对ExPASy蛋白质序列数据库进行检索,即获得差异表达的蛋白质。对鉴定出的部分蛋白质通过免疫组化方法进行验证。结果经双向凝胶电泳分析共有97个点被确定为差异表达,其中31个点已被成功鉴定,23个蛋白上调,8个蛋白下调。这些蛋白质涉及体内不同的功能过程和蛋白家族,主要包括:糖酵解相关酶,透明质酸酶,Ⅰ、Ⅱ相代谢酶,金属蛋白酶,细胞骨架,Ca2+代谢相关酶等。免疫组化方法进一步证实了HYAL1表达上调,CYP2C8和GSTM2表达下调。结论应用比较蛋白质组学方法可筛选出胰腺癌患者癌组织和正常胰腺组织间存在的差异表达蛋白,筛选出的蛋白将为进一步阐述胰腺癌的发生发展机制提供很好的线索和帮助。
[Abstract]:Objective to determine the difference of protein expression profiles between pancreatic cancer tissues and normal pancreatic tissues by differential proteomics, and to elucidate the relationship between the changes of protein expression levels and the occurrence and development of pancreatic cancer. Methods six pairs of pancreatic cancer tissues and corresponding normal pancreatic tissues were selected and total proteins were extracted by two-dimensional polyacrylamide gel electrophoresis. The differentially expressed protein spots were obtained by PDQuest software analysis. The differential protein spots were digested and hydrolyzed by electrospray quadrupole mass spectrometry (ESI-Q-TOF MS/MS). The MASCOT search engine was used to search the ExPASy protein sequence database and the differentially expressed proteins were obtained. Some of the identified proteins were verified by immunohistochemical method. Results A total of 97 sites were identified as differentially expressed by two-dimensional gel electrophoresis, 31 of which were successfully identified, 23 proteins were up-regulated and 8 proteins were down-regulated. These proteins are involved in different functional processes and protein families in vivo, including glycolytic enzymes, hyaluronidase, phase 鈪，
本文编号：2312383
[Abstract]:Objective to determine the difference of protein expression profiles between pancreatic cancer tissues and normal pancreatic tissues by differential proteomics, and to elucidate the relationship between the changes of protein expression levels and the occurrence and development of pancreatic cancer. Methods six pairs of pancreatic cancer tissues and corresponding normal pancreatic tissues were selected and total proteins were extracted by two-dimensional polyacrylamide gel electrophoresis. The differentially expressed protein spots were obtained by PDQuest software analysis. The differential protein spots were digested and hydrolyzed by electrospray quadrupole mass spectrometry (ESI-Q-TOF MS/MS). The MASCOT search engine was used to search the ExPASy protein sequence database and the differentially expressed proteins were obtained. Some of the identified proteins were verified by immunohistochemical method. Results A total of 97 sites were identified as differentially expressed by two-dimensional gel electrophoresis, 31 of which were successfully identified, 23 proteins were up-regulated and 8 proteins were down-regulated. These proteins are involved in different functional processes and protein families in vivo, including glycolytic enzymes, hyaluronidase, phase 鈪，
本文编号：2312383
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