水体细菌微生物多波长透射光谱解析模型

发布时间：2018-02-28 23:20

本文关键词： 光谱学多波长透射光谱 Mie散射细菌微生物解析模型　出处：《光学学报》2017年08期 　论文类型：期刊论文

【摘要】：细菌多波长透射光谱包含有细菌结构、组分、浓度等信息,这些特征信息的有效提取是实现细菌微生物快速识别与检测的基础。以水体常见的大肠埃希氏菌(大肠杆菌)为研究对象,采用紫外-可见分光光度法获得了其多波长透射光谱;基于Mie散射理论,在充分考虑水体大肠杆菌散射和吸收特性的基础上,构建了240~900nm波段范围内细菌微生物多波长透射光谱的解析模型;基于该模型对250~750nm特征波段范围内的光谱进行解析,获得了大肠杆菌的体积、粒径、结构及浓度等相关参数,并将这些参数与文献及实验得到的结果进行了对比验证。结果表明,建立的多波长透射光谱解析模型能够准确表征水体细菌微生物的光谱特征,该模型可为水体细菌微生物的快速识别分析和检测提供关键数据。
[Abstract]:The multiwavelength transmission spectrum of bacteria contains the information of bacterial structure, composition, concentration, etc. The effective extraction of these characteristic information is the basis for the rapid identification and detection of bacteria and microorganisms. The common Escherichia coli (E. coli) in water was used as the research object. The multiwavelength transmission spectra were obtained by UV-Vis spectrophotometry. Based on the Mie scattering theory, the scattering and absorption characteristics of Escherichia coli in water were fully considered. The analytical model of the multiwavelength transmission spectrum of bacterial microbes in the wavelength range of 240 ~ 900 nm was constructed, and based on the analytical model, the parameters such as volume, particle size, structure and concentration of Escherichia coli were obtained by analyzing the spectrum in the characteristic band range of 250 ~ 750nm. These parameters are compared with the results obtained from literature and experiments. The results show that the analytical model of multi-wavelength transmission spectrum can accurately characterize the spectral characteristics of bacteria and microorganisms in water body. The model can provide key data for rapid identification, analysis and detection of bacteria and microorganisms in water.
【作者单位】：中国科学院安徽光学精密机械研究所环境光学与技术重点实验室;中国科学技术大学;安徽省环境光学监测技术重点实验室;
【基金】：国家自然科学基金(61378041) 安徽省自然科学基金(1508085JGD02,1608085QF137) 安徽省科技重大专项(15CZZ04125)
【分类号】：O433.4;Q93

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