基于拉曼光谱对人舌鳞癌动物模型的建立与检测

发布时间：2018-04-24 13:25

本文选题：激光共焦显微拉曼光谱仪 + 人舌鳞癌细胞　；参考：《华南师范大学学报(自然科学版)》2017年04期

【摘要】：建立人舌鳞癌细胞体外癌变模型,采用激光共焦显微拉曼光谱仪对其进行检测,从分子层面对其进行分析.通过培养CAL-27细胞,然后注射在裸鼠皮下成瘤,采用激光共焦显微拉曼光谱仪和苏木精-伊红(HE)染色法观察其癌变的情况.结果表明,正常皮肤组织的切片清晰可见,其细胞层次清晰、整齐,但是肿瘤组织发现上皮异常增生,细胞多形性,结构层次非常不清楚;采用共焦显微拉曼光谱技术,结合主成分分析法和线性判别技术对健康裸鼠和人舌鳞癌细胞体外癌变组织进行分类,其得到诊断灵敏度为98.3%,特异性为95.0%,诊断正确率为96.7%.该方法可以很好地对该动物模型进行在体的检测.
[Abstract]:In vitro carcinogenesis model of human tongue squamous carcinoma cells was established. Laser confocal microscopic Raman spectroscopy was used to detect the cancer cells and to analyze them at the molecular level. CAL-27 cells were cultured and then injected into nude mice for subcutaneous tumorigenesis. Laser confocal microRaman spectroscopy and hematoxylin eosin (HE) staining were used to observe the carcinogenesis of CAL-27 cells. The results showed that the sections of normal skin tissue were clearly visible, and the levels of cells were clear and neat, but the tumor tissue found that the epithelial cells were hyperplastic, the cell pleomorphism and the structure levels were not clear. In combination with principal component analysis and linear discriminant technique, the cancerous tissues of healthy nude mice and human tongue squamous cancer cells were classified in vitro. The diagnostic sensitivity was 98.3, the specificity was 95.0, and the diagnostic accuracy was 96.7%. This method can be used to detect the animal model in vivo.
【作者单位】：华南师范大学生物光子学研究院国家中医药管理局中医药与光子技术三级实验室;暨南大学附属第一医院;
【基金】：国家自然科学基金项目(31300691,61675072,21505047,61335011,11404116) 教育部高等学校博士学科点专项科研基金项目(20134407120003) 广东省科技计划项目(2014A020212282) 广东省自然科学基金重点项目(9251063101000009) 广东省重大科技专项项目(2012A080203008) 广东省教育厅科技创新项目(2013KJCX0052)
【分类号】：R-332;R739.86

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