荧光偏振检测系统研究

发布时间：2018-12-27 09:48

【摘要】：荧光偏振检测系统是运用荧光偏振作为基本原理而开发设计的检测系统,该系统在医学医疗、生命科学、药物开发、环境监测等方面有着较为广泛的应用。论文将荧光偏振检测系统主要应用在生物医学检测以及环境保护中有机物分子的检测。荧光偏振检测原理是检测经过荧光探针标记的检测样本受激发光激发后所产生的发射光偏振强度的大小。样本受激发光照射后产生的特定波长的荧光经过发射滤光片和发射偏振片后变为偏振光进入光电倍增管,光电倍增管的作用是将垂直或者水平偏振光信号转化为微弱的电流信号,电流信号经过调理电路变为适当大小的模拟电压信号后经模数转换通过SPI接口输入到微处理器中,微处理器经过处理即可判断样品是否是待检测的物质。论文主要从两个方面进行设计：荧光偏振检测系统硬件设计和荧光偏振检测系统软件设计。荧光偏振检测系统软件设计分为系统功能程序开发和上位机软件开发。荧光偏振检测系统的硬件设计部分主要包括主控制电路、荧光检测电路、光源亮度检测电路、光源驱动电路、步进电机驱动电路、模数(A/D)转换电路、通信电路等,其中步进电机的驱动电路用以驱动步进电机进行试剂盘定位和在进行不同样品检测时所需要的不同光学镜片组合的选取以及在检测过程中为测取水平偏振光强度值和垂直偏振光强度值而分别进行的发射光路中水平偏振片和垂直偏振片的选取。软件部分的系统程序功能主要介绍了系统整体运行流程和荧光检测流程,上位机部分因不在本论文详细探讨范围之内,只对其功能进行了简单的介绍。所设计的荧光偏振检测系统经过各项实验测试验证,各项功能都达到了设计要求。
[Abstract]:The fluorescence polarization detection system is developed and designed by using fluorescence polarization as the basic principle. The system has been widely used in medical treatment, life science, drug development, environmental monitoring and so on. In this paper, fluorescence polarization detection system is mainly used in biomedical detection and detection of organic molecules in environmental protection. The principle of fluorescence polarization detection is to detect the polarization intensity of the emission light generated by the excitation of the sample labeled by the fluorescence probe. The fluorescence of a specific wavelength produced by the irradiation of the sample by excitation light passes through the emission filter and the emission polarizer, and then becomes polarized light into the photomultiplier tube, The function of photomultiplier tube is to convert vertical or horizontal polarized light signal into weak current signal. The current signal is transformed into analog voltage signal of appropriate size by conditioning circuit, and input to microprocessor via SPI interface. The microprocessor can be processed to determine whether the sample is a substance to be tested. The thesis mainly designs from two aspects: the hardware design of the fluorescence polarization detection system and the software design of the fluorescence polarization detection system. The software design of fluorescence polarization detection system is divided into system function program development and host computer software development. The hardware design of the fluorescence polarization detection system mainly includes the main control circuit, the fluorescence detection circuit, the light source brightness detection circuit, the light source driving circuit, the stepper motor driving circuit, the A / D conversion circuit, the communication circuit and so on. The driving circuit of the stepper motor is used to drive the step motor to locate the reagent plate, to select the different optical lens combination needed for different sample detection and to measure the horizontal polarized light intensity in the detection process. The horizontal polarizer and the vertical polarizer in the emission path are selected respectively. The system program function of the software part mainly introduces the whole operation flow and the fluorescence detection flow of the system. The upper computer part is not within the scope of the detailed discussion in this paper, only the function of the upper computer is simply introduced. The designed fluorescence polarization detection system is verified by various experiments and all functions meet the design requirements.
【学位授予单位】：西安工业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TH74

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