基于金属氧化物复合纳米结构的葡萄糖生物传感器的制备及性能研究

发布时间：2018-03-24 01:23

本文选题：Pt纳米颗粒　切入点：ZnO纳米线阵列　出处：《合肥工业大学》2016年硕士论文

【摘要】：本文以ZnO纳米线阵列和CeO2纳米线阵列作为研究基础对象,并对其进行修饰改性,采用戊二醛(GLA)和牛血清白蛋白(BSA)共交联的方法将葡萄糖氧化酶(GOx)负载于修饰改性后的纳米阵列表面制备葡萄糖生物传感器,分别对其性能进行研究。分别研究了基于Pt纳米颗粒修饰的ZnO纳米线阵列,CeO2纳米线阵列和碳修饰CeO2纳米线阵列复合结构的葡萄糖生物传感器的制备方法及其性能。此外,对于检测电位和交联液组成对传感器性能的影响做了研究。具体研究结果如下：首先,通过电化学沉积法优化沉积电位制备出高度有序的ZnO纳米线阵列,在此基础上采用水热法以氯铂酸为铂源,调节水热反应浓度和反应时间成功地在ZnO纳米线阵列上负载Pt纳米颗粒。采用交联法在纳米线阵列表面负载葡萄糖氧化酶,交联液的成分为5 v/v%GLA,4.8 w/v%BSA,300U/mL GOx。结合微流分析技术对所制备的葡萄糖生物传感器的性能进行研究,研究发现,基于流动注射分析的生物传感器对葡萄糖检测的灵敏度可达10.80μA·mM-1·cm-2。其次,采用水热法通过调节水热反应温度、时间等参数在泡沫镍上制备出高度有序的CeO2纳米线阵列。同样采用交联法在纳米线表面负载GOx,用传统的计时电流法对葡萄糖浓度进行检测。对传感器的检测电位、GOx浓度等进行优化,最终传感器表现出很高的灵敏度、较低的检测极限和较宽的检测范围等优势,灵敏度高达325.95μA·mM-1·cm-2。最后,在CeO2纳米线阵列的基础上,以葡萄糖作为碳源在CeO2纳米线阵列上负载上无定型碳,形成一种复合材料纳米结构,然后用GLA和BSA的共交联将GOx负载于碳修饰的CeO2纳米线阵列表面构筑葡萄糖生物传感器,其在-0.6 V的工作电位下,所制备的葡萄糖生物传感器对葡萄糖的检测灵敏度可达206.5 μA·mM-1·cm-2。通过对比研究发现：基于CeO2纳米线阵列的葡萄糖生物传感器在正电位下对葡萄糖具有较高的响应,但是其抗干扰能力较差；经过碳修饰之后的CeO2纳米线阵列在负电位下对葡萄糖具有较好的响应,同时具有较好的抗干扰性能。
[Abstract]:In this paper, ZnO nanowire array and CeO2 nanowire array are taken as the basic research objects and modified. Glucose biosensor was prepared by co-crosslinking glutaraldehyde (GLA) and bovine serum albumin (BSA) on the surface of modified nano-array. The preparation methods and properties of ZnO nanowire array based on Pt nanoparticles modified CeO2 nanowire array and carbon modified CeO2 nanowire array composite structure were studied. The effects of potential detection and cross-linking solution composition on the performance of the sensor are studied. The results are as follows: firstly, a highly ordered ZnO nanowire array is prepared by optimizing deposition potential by electrochemical deposition method. On this basis, Pt nanoparticles were successfully loaded on ZnO nanowire array by hydrothermal method with chloroplatinic acid as platinum source, and glucose oxidase was loaded on the surface of nanowire array by adjusting the concentration and reaction time of hydrothermal reaction. The composition of crosslinking solution is 5 v / v / v / 4 W / V BSA 300U / mL GOx.Combined with microflow analysis technique, the performance of the glucose biosensor was studied. It was found that the sensitivity of the biosensor based on flow injection analysis to glucose detection was 10.80 渭 A mM-1 / cm ~ (-2). Second, the sensitivity of glucose biosensor based on flow injection analysis was 10.80 渭 A mM-1 / cm ~ (-2). By adjusting the temperature of hydrothermal reaction by hydrothermal method, A highly ordered CeO2 nanowire array was prepared on nickel foam with time and other parameters. The glucose concentration on the surface of the nanowires was also measured by cross-linking method, and the glucose concentration was measured by the traditional chronoamperometric method. The detection potential of the sensor was obtained. The concentration of GOx is optimized, The sensitivity of the sensor is as high as 325.95 渭 A mM-1 cm ~ (-2). Finally, based on the CeO2 nanowire array, the sensor has the advantages of high sensitivity, low detection limit and wide detection range. Glucose was used as carbon source to load amorphous carbon onto CeO2 nanowire array to form a composite nanostructure. Then GOx was loaded on the surface of carbon-modified CeO2 nanowire array by co-crosslinking of GLA and BSA to construct glucose biosensor. At a working potential of -0.6 V, The glucose biosensor has a sensitivity of 206.5 渭 A mM-1 / cm ~ (-2). It is found that the glucose biosensor based on CeO2 nanowire array has a high response to glucose at positive potential. The carbon modified CeO2 nanowire array has a good response to glucose at negative potential and has good anti-interference performance.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TP212;TB383.1

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