一种超灵敏检测17β-雌二醇电化学核酸适配体传感器构建方法

发布时间：2018-04-17 10:43

本文选题：核酸适配体 + 石墨烯　；参考：《东华大学学报(自然科学版)》2017年05期

【摘要】：采用石墨烯(GS)-Nafion-纳米金(AuNPs)复合膜修饰玻碳电极(GCE/GS-Nafion-AuNPs),构建一种灵敏度高且稳定性好的电化学核酸适配体传感器,应用于环境内分泌干扰物——17β-雌二醇(E2)的快速检测.运用透射扫描电子显微镜(TEM)、循环伏安(CV)法和差分脉冲伏安(DPV)法,对该传感器的形貌和电化学性质进行表征.研究表明,在GS、Nafion和AuNPs协同作用下,修饰电极的电化学性能明显提高.在优化试验条件下,传感器的电流信号(I_p)与17β-雌二醇(E2)的浓度(c)的对数在0.002 5~0.300 0μmol/L范围内呈现良好的线性关系,回归方程为I_p=8.899 4+1.049 4logc,相关系数为0.994 1,E2浓度检出限为0.83nmol/L.该传感器制作简单、灵敏度高、检出限极低以及具有良好的重现性和稳定性,有望应用于环境样品中E2的检测.
[Abstract]:A novel electrochemical aptamer sensor with high sensitivity and good stability was constructed by modified glassy carbon electrode (GCE-Nafion-AuNPsN) with GCE-Nafion-Nano-AuNPs composite membrane, which was used for rapid detection of environmental endocrine disrupt-17 尾 -estradiol (17 尾 -estradiol).The morphology and electrochemical properties of the sensor were characterized by transmission scanning electron microscopy (TEM), cyclic voltammetry (CV) and differential pulse voltammetry (DPV).The results showed that the electrochemical performance of the modified electrode was improved obviously under the synergistic action of GSN Nafion and AuNPs.Under the optimized test conditions, the logarithm of the current signal of the sensor is linear with the concentration of 17 尾 -estradiol E2) in the range of 0.002 渭 mol/L. The regression equation is I_p=8.899 4 1.049 4 logc, and the correlation coefficient is 0.994 1 渭 mol / L of E2 concentration.The sensor has the advantages of simple fabrication, high sensitivity, low detection limit and good reproducibility and stability. It is expected to be applied to the detection of E2 in environmental samples.
【作者单位】：东华大学环境科学与工程学院;东华大学国家环境保护纺织污染防治工程技术中心;
【基金】：国家自然科学基金资助项目(21377033,51508083) 中央高校基本科研业务费专项资金资助项目(2232015D3-22) 上海市重点学科建设资助项目(B604)
【分类号】：O657.1;TP212

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