低温等离子体对小鼠皮肤伤口愈合的影响

发布时间：2017-12-28 08:47

本文关键词：低温等离子体对小鼠皮肤伤口愈合的影响　出处：《西安交通大学学报(医学版)》2017年05期 　论文类型：期刊论文

更多相关文章： 等离子体射流 BALB/c小鼠 创伤愈合

【摘要】：目的比较不同剂量的大气压低温等离子体对BALB/c小鼠伤口愈合的影响,探讨低温等离子体处理小鼠伤口的最佳剂量以及影响伤口愈合的机制。方法采用介质阻挡放电方法产生大气压低温等离子体射流,对小鼠皮肤伤口进行处理,按处理时间分为10s、20s、30s、40s、50s实验组,以不处理的自然愈合的伤口作为阴性对照组,以人重组表皮生长因子处理伤口作为阳性对照组,每天处理1次,连续观察14d。每天记录各组的伤口尺寸变化;取处理第7天的伤口组织行HE染色观察组织病理学变化;免疫荧光染色技术检测伤口组织中Ⅰ型胶原蛋白的表达;发射光谱分析低温等离子体射流的成分。结果 10s、20s、30s、40s实验组的小鼠伤口愈合速度较快,分别在第12、10、7、13天均基本愈合,而50s实验组的小鼠伤口在第14天尚未愈合;阳性对照组的小鼠伤口在第7天全部愈合,30s实验组可以达到阳性对照组小鼠伤口愈合效果。HE染色与免疫荧光染色技术表明,30s的等离子体处理剂量可以最大限度的促进伤口表皮再生,肉芽组织增生和胶原沉淀。光谱诊断发现低温等离子体中含有OH、NO、激发态N2分子以及激发态O原子。结论适量的低温等离子体可促进小鼠的伤口愈合,而过量则会抑制小鼠伤口的愈合;伤口的愈合过程可能和低温等离子体中的活性氧、活性氮的作用相关。
[Abstract]:Objective to compare the effects of different doses of atmospheric pressure and low temperature plasma on wound healing in BALB/c mice, and to explore the best dose of low temperature plasma treatment of wound and the mechanism of wound healing. Methods using atmospheric pressure plasma jet generated by DBD, mice were treated with skin wounds, according to the processing time is divided into 10s, 20s, 30s, 40s, 50s in the experimental group, no treatment of natural healing wounds as the negative control group with recombinant human epidermal growth factor in wound treatment as a positive control group. 1 times a day, continuous observation of 14D. The wound size was recorded every day. The wound tissues were treated for seventh days. HE staining was used to observe histopathological changes. The expression of type I collagen in wound tissues was detected by immunofluorescence staining, and the constituents of low temperature plasma jet were analyzed by emission spectrum. The results of 10s, 20s, 30s in wound healing, 40s group faster, respectively at twelfth, tenth, seventh and 13 days were healed, and the mice wound 50s experimental group in Fourteenth days yet healing; wound in mice of positive control group healed in seventh days, the experimental group 30s was positive control group of mice wound healing effect. HE staining and immunofluorescence staining showed that the plasma treatment dose of 30s could maximize wound epidermal regeneration, granulation tissue proliferation and collagen deposition. It is found that the low temperature plasma contains OH, NO, excited state N2 molecules and excited state O atoms in the low temperature plasma. Conclusion a proper amount of low temperature plasma can promote wound healing in mice, while excess will inhibit wound healing in mice. Wound healing may be related to reactive oxygen and reactive nitrogen in cryogenic plasma.
【作者单位】：西安交通大学公共卫生学院卫生毒理与卫生检验系;西安交通大学电力设备电气绝缘国家重点实验室;陕西省疾病预防控制中心消毒与病媒生物防控所;
【基金】：国家自然科学基金资助项目(No.81372076、51677146) 陕西省科技计划项目(No.2010K16-04)~~
【分类号】：R45
【正文快照】： Supported by the National Natural Science Foundation of China(No.81372076 and 51677146)and the Sci-Tech Project of ShaanxiProvince(No.2010K16-04)近年来,低温等离子体(low-temperature plas-ma,LTP)处理体外培养的细胞和活体组织的效果及机制已成为等离子体医学领

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