多耐药鲍曼不动杆菌噬菌体D218的生物学特性研究

发布时间：2017-12-28 09:08

本文关键词：多耐药鲍曼不动杆菌噬菌体D218的生物学特性研究　出处：《国际检验医学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的筛选可用于裂解多耐药鲍曼不动杆菌的噬菌体,为噬菌体治疗多耐药鲍曼不动杆菌治疗提供新的方案。方法以上海各家医院临床分离到的多耐药鲍曼不动杆菌为宿主菌,从不同环境中分离鲍曼不动杆菌噬菌体,负染法电镜观察其形态及大小,并分析pH和温度对其稳定性的影响,并对噬菌体DNA进行测序及相关基因功能分析。结果从医院下水道污水中分离得到了一株耐药性鲍曼不动杆菌的烈性噬菌体D218,电镜观察其为长尾噬菌体,头部呈多面体立体对称结构,直径约65nm,尾长约100nm,尾宽约15nm。此株噬菌体在pH 3~11及4~50℃的环境下具有较高活性。其基因组全长102 449bp,GC含量为37.2%,编码167个ORFs。结论筛选到了一株新的对pH值及温度均稳定的耐药鲍曼不动杆菌烈性噬菌体D218,为噬菌体用于耐药性鲍曼不动杆菌的院内感染的预防控制及噬菌体库的建立增添了新的数据。
[Abstract]:Objective to screen the bacteriophages that can be used for lysate multidrug resistant Acinetobacter Bauman and to provide a new method for the treatment of multidrug resistant Acinetobacter and Acinetobacter. In the treatment of bacteriophage. Methods to multi drug resistant Bauman Shanghai hospitals clinical isolates of Acinetobacter as host bacterium isolated Bauman Acinetobacter phage from different environment, observe the morphology and size of negative staining electron microscopy, and analysis of the influence of pH and temperature on the stability, and the phage DNA was analyzed by sequencing and gene function. Results a virulent bacteriophage D218 of Acinetobacter baumannii was isolated from hospital sewage and sewer. The electron microscope showed that it was a long tail phage. The head had a polyhedron symmetrical structure. The diameter was about 65nm, the tail length was about 100nm, and the tail width was about 15nm. The phage has high activity in the environment of pH 3~11 and 4~50 C. The total length of the genome is 102 449bp, the content of GC is 37.2%, and the encoding is 167 ORFs. Conclusion a new D218 resistant Acinetobacter baumannii strain D218, which is stable against pH and temperature, has been screened. It provides new data for phage control and prevention of nosocomial infection caused by Acinetobacter Bauman, and the establishment of phage library.
【作者单位】：上海市徐汇区中心医院;上海交通大学医学院附属新华医院崇明分院;上海交通大学医学院附属瑞金医院烧伤科;复旦大学附属妇产科医院检验科;
【基金】：上海市科学技术委员会自然科学基金资助项目(16ZR1420800) 上海青年临床医技人才(临床检验专业)培养资助计划资助项目(沪医卫基[2016]04号)
【分类号】：R446.5;R440
【正文快照】： 鲍曼不动杆菌在自然界环境中广泛分布,常存在于人或动物体表、泌尿生殖道和呼吸道,是引起严重院内感染最常见的条件致病菌[1]。据2015年中国耐药检测网(CHINET)数据显示,鲍曼不动杆菌对碳青霉烯类的亚胺培南或美罗培南的耐药率全国为59.0%,各省鲍曼不动杆菌对碳青霉烯类耐药率

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