miR-132在神经病理性疼痛传导通路中的表达

发布时间：2017-12-31 19:24

本文关键词：miR-132在神经病理性疼痛传导通路中的表达　出处：《实用医学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的通过机械损伤致神经病理痛大鼠模型,检测神经系统不同节段mi R-132定量变化。方法 45只SD大鼠随机平均分为对照组(Native)、假手术组(Shame)和坐骨神经结扎组(BCCI),每组15只。于术前1 d,术后1、7、14 d测定机械痛阈(MWT)。取海马,L4~6脊髓背角、背根神经节并采用RT-PCR定量检测mi R-132的表达。结果 BCCI组MWT在术后7和14 d明显低于Native组及Shame组(P0.01)。Mi R-132表达量:(1)背根神经节:术后第7天BCCI组和Shame组相比Native组均明显降低(P0.05);第14天Shame组恢复至术前水平,而BCCI组表达量仍降低(P0.05)。(2)脊髓背角:术后第7天BCCI组表达量明显增高(P0.05),第14天BCCI组与Shame组均明显降低(P0.05)。(3)海马:术后第14天BCCI组表达量明显降低(P0.01)。结论 mi RNA-132参与了损伤性神经病理痛的发生发展。mi RNA-132在BCCI大鼠模型的疼痛传导通路不同节段不同时间节点表达水平发生显著的变化,不同部位的变化存在明显的差异性。
[Abstract]:Objective to detect the quantitative changes of mi R-132 in different segments of nervous system in rats with neuropathic pain induced by mechanical injury. Methods 45 SD rats were randomly divided into control group (n = 45). In sham-operated group and sciatic nerve ligation group, 15 rats in each group were used to measure the mechanical pain threshold (MWTT) at 1 day before operation and 1 day after operation. The hippocampus was taken from the hippocampus. L4 and 6 dorsal horn of spinal cord. Results MWT in BCCI group was significantly lower than that in Native group and Shame group at 7 and 14 days after operation (P < 0.05). The expression of P0.01C. Mi R-132 in dorsal root ganglion (DRG): on the 7th day after operation, the expression of P0.05 in BCCI group and Shame group was significantly lower than that in Native group. On the 14th day, the Shame group recovered to the preoperative level. However, the expression level of BCCI group was still lower than that of the control group (P 0.05, P 0.05). The expression of BCCI in the BCCI group was significantly higher than that in the control group on the 7th day after operation (P 0.05). On the 14th day, both BCCI group and Shame group significantly decreased the expression of P0.05 and P0.01 in hippocampus: on the 14th day after operation, the expression of BCCI decreased significantly in BCCI group (P 0.01). Conclusion mi RNA-132 is involved in the occurrence and development of injured neuropathic pain. Mi. The expression level of RNA-132 in different segments of pain conduction pathway in BCCI rats was significantly changed at different time points. There are obvious differences in the changes of different parts.
【作者单位】：南京医科大学附属上海松江中心医院;
【基金】：上海市松江区科委科技攻关项目(编号:14SJG-Gyy10)
【分类号】：R402
【正文快照】： 神经病理性疼痛(neuropathic pain,NPP)是由躯体感觉神经系统的损伤或者疾病所引起的疾病。其发病机制复杂,涉及病理过程繁多,伴有痛觉传导通路不同部位基因表达的改变。在神经系统,mi RNAs参与神经系统的分化、神经元的存活、轴突的生长、脊髓的形成过程;另一方面在疼痛的发

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