MALDI-TOFMS技术在鉴定临床少见非发酵菌中的应用

发布时间：2017-12-31 23:19

本文关键词：MALDI-TOFMS技术在鉴定临床少见非发酵菌中的应用　出处：《中国现代医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的评价基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)技术在快速鉴定临床少见非发酵菌中的应用价值。方法选取2013年7月-2016年10月安徽医科大学第一附属医院检验科分离的67株临床少见非发酵菌,包括痰液分离的21株,尿液分离的13株,血液分离的9株,骨髓液分离的9株,分泌物分离的8株,腹透液分离的2株,脑脊液分离的1株,胆汁分离的1株,其他体液分离的3株。以16S核糖体RNA(16SrRNA)序列分析为金标准,比较MALDI-TOF MS与Vitek2 Compact鉴定系统鉴定的准确性。结果 67株临床少见非发酵菌中,MALDI-TOF MS将66株(98.5%)鉴定到种水平,1株(1.5%)鉴定到属水平;Vitek2Compact鉴定系统将59株(88.1%)鉴定到种水平,8株(11.9%)未能鉴定;67株少见非发酵菌均扩增到16SrRNA目的基因片段并成功测序,67株(100.0%)都成功鉴定到种水平。以16SrRNA序列分析为金标准,MALDITOF MS和Vitek2 Compact鉴定系统正确鉴定到种水平准确性分别为98.5%(66/67)和88.1%(59/67)。结论与传统的细菌生化反应方法相比,MALDI-TOF MS技术操作简便、耗时短、费用低,鉴定结果与16SrRNA序列分析的符合率高,可以提高临床对少见非发酵菌鉴定的准确率。
[Abstract]:Objective to evaluate MALDI-TOF MS by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF). Methods from July 2013 to October 2016, 67 strains of clinical rare non-fermentative bacteria isolated from the laboratory of the first affiliated hospital of Anhui medical university were selected for rapid identification of clinical non-fermentative bacteria. Bacteria. There were 21 strains of sputum, 13 strains of urine, 9 strains of blood, 9 strains of bone marrow fluid, 8 strains of secretions, 2 strains of peritoneal dialysis and 1 strain of cerebrospinal fluid. 1 strain isolated from bile and 3 strains isolated from other humoral fluids. The 16s ribosomal RNAs 16s rRNA sequence analysis was regarded as the gold standard. The accuracy of MALDI-TOF MS and Vitek2 Compact identification system was compared. MALDI-TOF MS identified 66 plants at the seed level and 1 strain at the genus level. Vitek2Compact identification system identified 59 strains (88. 1) to the seed level, 8 (11. 9) could not be identified; The 16s rRNA target gene fragment was amplified from 67 strains of non-fermentative bacteria and 67 strains were sequenced successfully. The 16s rRNA sequence analysis was regarded as the gold standard. The accuracy of MALDITOF MS and Vitek2 Compact identification system was 98.566 / 67 and 88.1 / 59 / 67, respectively. Conclusion compared with the traditional method of bacterial biochemical reaction. MALDI-TOF MS technique is simple, time consuming and low cost. The result of identification has a high coincidence rate with 16s rRNA sequence analysis, which can improve the accuracy of clinical identification of rare non-fermentative bacteria.
【作者单位】：安徽医科大学第一附属医院检验科;
【分类号】：R446.5
【正文快照】： 临床上分离的常见非发酵菌以铜绿假单胞菌、(美国Sigma公司),-80℃超低温冰箱(日本SANYO鲍曼不动杆菌等为主[1-2],对较少分离的非发酵菌目公司)。前用传统的生化反应方法鉴定,影响因素较多,鉴定1.3质控和校准菌株结果不理想。基质辅助激光解析电离飞行时间质谱质控:大肠埃希菌

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