抗菌肽17BIPHE2对金黄色葡萄球菌生物被膜的抑制作用

发布时间：2018-01-02 08:46

本文关键词：抗菌肽17BIPHE2对金黄色葡萄球菌生物被膜的抑制作用　出处：《微生物学通报》2017年04期 　论文类型：期刊论文

【摘要】：【目的】研究抗菌肽17BIPHE2单独使用及联合抗生素对金黄色葡萄球菌(Staphylococcus aureus)生物被膜的抑制作用。【方法】采用刚果红平板测试法和结晶紫染色评估受试菌形成生物被膜的能力;微量肉汤稀释法和琼脂平板测试法测定金黄色葡萄球菌最小抑菌浓度(MIC)和最小杀菌浓度(MBC);利用抑制金黄色葡萄球菌黏附实验和生物被膜形成抑制实验观察17BIPHE2单独使用及联合抗生素对生物被膜黏附阶段和形成阶段的影响;通过扫描电子显微镜(SEM)观察17BIPHE2单独使用及联合抗生素对成熟生物被膜的清除作用。【结果】17BIPHE2的MIC为8μmol/L,1/2×MIC就可以有效抑制浮游菌的生长。单独使用17BIPHE2在细菌黏附阶段抑制率为40%,在生物被膜形成阶段抑制率达到35%。17BIPHE2联合抗生素使用较单独使用抗生素其抑制率均有所下降。生物被膜成熟阶段17BIPHE2于1/4×MIC浓度即可促进生物被膜崩解,1×MIC生物被膜崩解同时细菌黏附量有所下降,联合万古霉素促进生物被膜崩解同时细菌胞质大量外泄。【结论】抗菌肽17BIPHE2具有良好的抑制金黄色葡萄球菌生物被膜作用,联合抗生素其抗生物被膜作用进一步提高。这将为治疗由金黄色葡萄球菌生物被膜引起的相关感染提供了一个新思路。
[Abstract]:[Objective] to study the antibacterial peptide 17BIPHE2 used alone and combined with antibiotics against Staphylococcus aureus (Staphylococcus aureus) was inhibited biological membranes. [method] violet staining was assessed from the ability to form biofilm by Congo red plate test method and crystallization; Determination of the minimum inhibitory concentration of Staphylococcus aureus broth dilution method and agar plate test (MIC) and minimum bactericidal concentration (MBC); inhibit Staphylococcus aureus adhesion and biofilm formation inhibition observed with 17BIPHE2 alone and combined with antibiotics on the biological membrane adhesion stage and formation stage using; by scanning electron microscopy (SEM) observation of 17BIPHE2 alone and combined mature creature is the scavenging effect of antibiotics on membrane. [result] 17BIPHE2 MIC 8 mol/L, 1/2 * MIC can effectively inhibit the growth of planktonic bacteria alone. With 17BIPHE2 in the stage of bacterial adhesion inhibition rate was 40%, in the stage of biofilm formation inhibition rate of 35%.17BIPHE2 combined with the use of antibiotics with antibiotics alone and the inhibitory rate was decreased. The biofilm maturation stage 17BIPHE2 to 1/4 * MIC concentration can promote biofilm disintegration, 1 * MIC biofilm disintegration and bacterial adhesion the decline in the volume, combined with vancomycin promote biofilm disintegration and bacterial cytoplasmic large leakage. [Conclusion] the antibacterial peptide 17BIPHE2 has good inhibition of Staphylococcus aureus biofilms, the antibiotic combined with antibiotic biofitm effect further improved. This will provide a new way of thinking for the treatment of infection by Staphylococcus aureus biofilm caused.

【作者单位】：宁夏医科大学临床医学院检验系;宁夏医科大学科技中心;
【基金】：国家自然科学基金项目(No.31360622) 宁夏临床病原微生物重点实验室开放课题项目(No.LCPM201501)~~
【分类号】：R446.5
【正文快照】： 金黄色葡萄球菌(Staphylococcus aureus)是一种革兰氏阳性菌,广泛分布于自然界、人类体表及与外界相通的腔道中,约1/3的健康人群稳定携带该细菌[1]。同时,金黄色葡萄球菌是一种常见的人畜共患病病原菌,具有较高的发病率和死亡率,常可引起局部皮肤、组织、器官的化脓性感染及食

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