北京世纪坛医院亚胺培南耐药肺炎克雷伯菌院内感染流行病学调查

发布时间：2018-01-05 08:25

本文关键词：北京世纪坛医院亚胺培南耐药肺炎克雷伯菌院内感染流行病学调查　出处：《重庆医学》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨临床分离亚胺培南耐药肺炎克雷伯菌菌株间同源性关系及耐药菌可能的传播途径,为临床该类菌的传播控制提供依据。方法常规进行临床样本采集及细菌培养;同时对ICU病房医护人员手表面及患者床旁物品表面采样并做细菌计数及培养;细菌鉴定及药敏测定采用VITEK-2COMPACT全自动细菌鉴定/药敏分析系统;细菌同源性测定采用肠杆菌科基因组内重复序列聚合酶链反应(ERIC-rep-PCR)法。结果 2011年1月至2013年12月临床共分离到642株亚胺培南耐药肺炎克雷伯菌,菌株来源为ICU 389株(60.59%);呼吸内科72株(11.21%);神经内科53株(8.26%);心血管科41株(6.39%);外科31株(4.83%);消化内科11株(1.71%);肾脏内科5株(0.78%);急诊科17株(2.65%);其他科室23株(3.58%)。ICU物体表面分离到1株亚胺培南耐药肺炎克雷伯菌。642株临床菌株中含8个主要克隆,该8个克隆菌合计394株,占全部菌株的61.37%,其余248株菌为单一克隆。结论亚胺培南耐药的肺炎克雷伯菌在该院确实存在单克隆流行,接触传播可能是此类感染的传播途径。
[Abstract]:Objective to investigate the clinical isolates of imipenem resistant Klebsiella pneumoniae strains homologous transmission sex and drug resistant bacteria may provide the basis for clinical, spread the bacteria control. Methods routine clinical samples and bacterial culture; at the same time in ICU ward in the hands of medical personnel and patients bedside surface sampling and bacterial surfaces count and culture; identification of bacteria and drug sensitivity was determined by using VITEK-2COMPACT automatic bacteria identification and susceptibility analysis system; bacterial homology was determined by repeat polymerase chain reaction of Enterobacteriaceae genome (ERIC-rep-PCR) method. Results from January 2011 to December 2013 a total of 642 clinical isolated strains of imipenem resistant Klebsiella pneumoniae strains from 389 strains of ICU (60.59%); 72 (11.21%) strains of respiratory medicine; Department of Neurology; 53 strains (8.26%), 41 (6.39%) strains of cardiovascular surgery; 31 strains (4.83%); Department of Gastroenterology, 11 strains (1.71% Department of Nephrology); 5 strains (0.78%); emergency department of 17 strains (2.65%); other sections of 23 strains (3.58%).ICU surface containing 8 major clones isolated from 1 strains of imipenem resistant Klebsiella pneumoniae.642 strains, the 8 clones of bacteria total 394 strains, accounting for all strains 61.37%, the other 248 strains as a single clone. Conclusion Klebsiella pneumoniae resistant to imipenem exists monoclonal popular in the hospital, contact may be transmitted this kind of infection.

【作者单位】：首都医科大学附属北京世纪坛医院中心实验室;首都医科大学附属北京世纪坛医院临床检验中心;
【分类号】：R446.5
【正文快照】： 肺炎克雷伯菌是医院内感染常见病原菌,尤其是近年来对碳青霉烯类耐药肺炎克雷伯菌在医院的出现及流行,对抗感染治疗提出了新的挑战。了解亚胺培南耐药株在院内的传播机制,对可能的传播途径进行重点防控,将有助于控制此类感染在院内的传播流行[1]。本研究对临床分离的亚胺培南

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