多重PCR液相芯片技术在腹泻病原体检测中的应用

发布时间：2018-01-08 14:21

本文关键词：多重PCR液相芯片技术在腹泻病原体检测中的应用　出处：《现代预防医学》2017年18期 　论文类型：期刊论文

【摘要】：目的探讨多重PCR液相芯片技术检测腹泻病原体的可行性,为突发公共卫生事件处置提供实验依据。方法采用x TAGRGastrointestinal Pathogen Panel(x TAG汶 GPP)对模拟腹泻阳性样本和783份腹泻疫情样本进行检测,结果与常规检测法比较。结果模拟腹泻阳性样本均检出15种腹泻病原体。783份腹泻疫情标本中未检出艰难梭状芽胞杆菌(Clostridium difficile,C.difficile)、大肠埃希菌O157(Escherichia coli O157,E.coli O157)、微小隐孢子虫(Cryptosporidium,C.parvum)、痢疾阿米巴(Entamoeba histolytica,E.histolytica)和贾第虫(Giardia,Giar)。病毒和细菌检出率分别为55.04%和38.19%;诺如病毒GI/GII型(Norovirus GI/GII,NV GI/GII)、轮状病毒A型(Rotavirus A,RV A)、空肠弯曲菌(Campylobacter jejuni,C.jejuni)、肠毒素性大肠埃希菌LT/ST型(Enterotoxigenic E.coli LT/ST,ETEC)、志贺氏菌(Shigella,Shig)、产志贺毒素大肠埃希菌stx1/stx2型(Shiga-like Toxin producing E.coli stx 1/stx 2,STEC)、小肠结肠炎耶尔森菌(Yersinia enterocolitica,Y.enterocolitica)检出率差异均有统计学意义。结论多重PCR液相芯片技术具有高通量、高灵敏度、高特异性等优点,能较全面评估腹泻病原体和检测混合感染样本,处理好经费和PCR污染的问题,该方法可用于腹泻疫情的快速应急检测。
[Abstract]:Objective to investigate the feasibility of multiple PCR Liquichip detection of diarrhea pathogens, and provide experimental basis for the disposal of public health emergencies. Methods x TAGRGastrointestinal Pathogen (Panel x TAG GPP to simulate Wen) positive samples and 783 samples of diarrhea diarrhea samples. Results compared with the conventional detection method. The simulation results of positive samples were not detected in diarrhea Clostridium difficile diarrhea pathogens were detected in 15.783 samples of diarrhea epidemic (Clostridium difficile, C.difficile), Escherichia coli O157 (Escherichia coli O157, E.coli O157), Cryptosporidium parvum (Cryptosporidium, C.parvum), Amiba (Entamoeba histolytica, E.histolytica dysentery) and Giardia (Giardia, Giar). Viruses and bacteria detection rates were 55.04% and 38.19%; norovirus GI/GII (Norovirus GI/GII, NV GI/GII), rotavirus type A (Rotavirus A, RV A (Campylobacter), Campylobacter jejuni, jejuni, C.jejuni) of enterotoxigenic Escherichia coli LT/ST (Enterotoxigenic E.coli LT/ST, ETEC), Shigella (Shigella, Shig), Shiga toxin producing Escherichia coli stx1/stx2 (Shiga-like Toxin producing E.coli STX 1/stx 2, STEC), enterocolitis bacteria Jerson (Yersinia enterocolitica, Y.enterocolitica) detection rate differences were statistically significant. Conclusion multiple PCR liquid chip technology with high throughput, high sensitivity, high specificity, can comprehensively assess diarrhea pathogens and detect mixed infection samples, handle the funds and PCR pollution problems, this method can be used for fast detection of diarrhea epidemic emergency.

【作者单位】：常州市疾病预防控制中心;
【基金】：常州市科技支撑计划(社会发展)(项目号:CE20165042) 江苏省基础研究计划(自然科学基金)--青年基金项目(项目号:BK20150250)
【分类号】：R440
【正文快照】： 突发公共卫生事件的应急处理是疾病预防控制中心的重要工作之一,而实验室检测结果可为其流行病学调查、疫情控制和医疗救治提供决定性的证据。近年来,世界卫生组织报道腹泻是造成5岁以下儿童死亡的第二大原因,每年约有76万5岁以下儿童死于腹泻,全球每年约有17亿例腹泻病例[1]

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