低频电磁场对毛发生长及相关因子表达影响的实验研究

发布时间：2018-01-11 11:26

本文关键词：低频电磁场对毛发生长及相关因子表达影响的实验研究　出处：《南方医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的观察低频电磁场在毛发生长周期小鼠模型中对毛发生长及KGF、TGF-β1、PDGF-BB及FGFR2表达的影响。方法将120只毛发自然生长模型C57BL/6小鼠随机分成磁场组和对照组,每组60只。其中,磁场组小鼠选择低频电磁场(50 Hz、5mT)暴露,30min/d,连续干预14天;对照组不进行磁场处理,但饲养条件与磁场组一致。实验过程中,分别在磁场处理后的第3d、7d、14d留取小鼠背部皮肤组织。肉眼观察毛发生长差异;HE染色观察毛发及表皮组织形态;荧光定量PCR法检测KGF、TGF-β1、PDGF-BB 及 FGFR2 表达;Western Blot 检测 KGF、TGF-β1、PDGF-BB 及 FGFR2蛋白表达。结果1、肉眼观察,3d时可见两组小鼠背部毛发呈黑色,色泽鲜亮、浓密;7d时毛发逐渐脱落,变稀疏;至14d时,两组毛发几乎脱落完,脱毛处皮肤变粉红。第3d、7d时,磁场组和对照组中小鼠的毛发生长情况无明显差异。2、HE染色观察:与对照组相比,磁场干预后第3d、7d,小鼠毛发长度增加(P0.05),同时小鼠表皮厚度也增厚(P0.05);当磁场干预后第14天时,毛发生长进入退行期,与对照组相比较,其表皮厚度较厚。在干预过程中,对照组和磁场组的生长周期无明显差别。3、PCR检测结果:在磁场组和对照组中,组内第3d、7d、14d时间点中的KGF的表达逐渐降低,但变化差异无统计学意义(P0.05);与对照组相比,同时间点的磁场组KGFmRNA的表达增加,差异具有统计学意义(P0.05)。在对照组中,第3d、7d、14d时间点中的TGF-β1的表达逐渐增加,但变化差异无统计学意义(P0.05),而磁场组是先增加后降低,差异也无统计学意义(P0.05);与对照组相比,3d、7d磁场组TGF-β1的表达均增加,14d时磁场组低于对照组,差异无统计学意义(P0.05)。在磁场组和对照组中,组内第3d、7d、14d时间点中的PDGF-BB的表达是先增加后减少,但变化差异无统计学意义(P0.05);3d、7d两组的表达均高于14d(P0.05),与对照组相比,同时间点的磁场组PDGF-BB的表达下降,差异不具有统计学意义(P0.05)。在磁场组和对照组中,组内第3d、7d、14d时间点中的FGFR2的表达逐渐降低,但变化差异无统计学意义(P0.05);与对照组相比,同时间点的磁场组FGFR2的表达增加,差异不具有统计学意义(P0.05)。4、Western-blot检测结果:每个时间点KGF蛋白的表达磁场组较对照组均稍有增加,对照组KGF蛋白表达随时间变化不大,磁场组在7d时KGF蛋白表达较3d、14d的均高。在每个时间点TGF-β1的蛋白表达均是磁场组高于对照组;而两组的TGF-β1的蛋白表达随时间变化不大。在每个时间点,PDGF-BB的表达均是磁场组高于对照组;对照组在3d和7d时表达变化不大,但到14d时表达减小,而磁场组在7d时表达最多。FGFR2在3d、7d时的蛋白表达均是磁场组高于对照组,14d时两组表达差异不明显。结论低频电磁场可促进生长周期中小鼠毛发的生长;在该过程中,低频电磁场促进KGF、TGF-β1、PDGF-BB及FGFR2的表达。
[Abstract]:Objective to observe the effect of low frequency electromagnetic field on hair growth and KGF- 尾 1 in mouse hair growth cycle. Methods 120 C57BL / 6 mice were randomly divided into magnetic field group and control group. The mice in the magnetic field group were exposed to low frequency electromagnetic field (50 Hz) for 30 min / d for 14 days. The control group was not treated with magnetic field, but the feeding condition was the same as that in the magnetic field group. During the experiment, the skin tissue of the back of the mice was taken on the 3rd day, 7 days and 14 days after the magnetic field treatment, and the difference of hair growth was observed with naked eyes. The morphology of hair and epidermis was observed by HE staining. The expression of PDGF-BB and FGFR2 was detected by fluorescence quantitative PCR. The expression of PDGF-BB and FGFR2 protein was detected by Western Blot. On the 3rd day, the hair on the back of the two groups was black, bright and dense. At 7 days, the hair gradually fell off and became sparse. At day 14, the hair of the two groups was almost lost, and the skin became pink at the point of depilation. On the 3rd day, there was no significant difference in hair growth between the magnetic field group and the control group. Observation of HE staining: compared with the control group, the length of hair and the thickness of epidermis of mice were increased on the 3rd day and 7th day after the treatment of magnetic field, and the thickness of epidermis was also increased. At the 14th day after the magnetic field intervention, the hair growth entered into the retreat period, compared with the control group, the thickness of the epidermis was thicker. In the intervention process, the growth cycle of the control group and the magnetic field group was not significantly different. 3. The results of PCR detection showed that the expression of KGF in the magnetic field group and the control group decreased gradually at the 14th day after the 3rd day, but there was no significant difference between the two groups (P 0.05). Compared with the control group, the expression of KGFmRNA increased in the magnetic field group at the same time point, and the difference was statistically significant (P 0.05). The expression of TGF- 尾 1 increased gradually at 14d, but there was no significant difference in the change of TGF- 尾 1 expression, while in the magnetic field group, the expression of TGF- 尾 1 increased first and then decreased, and the difference was not statistically significant. Compared with the control group, the expression of TGF- 尾 _ 1 in the magnetic field group was significantly higher than that in the control group on the 3rd day and 7th day, and the expression of TGF- 尾 _ 1 in the magnetic field group was lower than that in the control group at the 14th day, and the difference was not statistically significant (P 0.05). The expression of PDGF-BB was increased first and then decreased at the 14th day after the 3rd day, but there was no significant difference between the two groups (P 0.05). The expression of PDGF-BB in the magnetic field group at the same time point was lower than that in the control group. The difference was not statistically significant (P 0.05). In the magnetic field group and the control group, the expression of FGFR2 decreased gradually at the 14th day after the 3rd day. But the difference was not statistically significant (P 0.05). Compared with the control group, the expression of FGFR2 in the magnetic field group at the same time point was increased, but the difference was not statistically significant (P 0.05). Western-blot results: the expression of KGF protein in the magnetic field group was slightly higher than that in the control group at each time point, but the expression of KGF protein in the control group did not change with time. The expression of KGF protein in the magnetic field group was higher than that in the control group on the 7th day, and the expression of TGF- 尾 1 protein in the magnetic field group was higher than that in the control group at each time point. However, the expression of TGF- 尾 1 protein in the two groups did not change with time. The expression of PDGF-BB in the magnetic field group was higher than that in the control group at each time point. The expression of FGFR2 in the magnetic field group was significantly higher than that in the control group on the 3rd day and 7th day in the magnetic field group, but the expression of FGFR2 in the magnetic field group was higher than that in the control group at the 3rd and 7th day, but at the 14th day, the expression of the highest expression of FGFR2 in the magnetic field group was higher. Conclusion the low frequency electromagnetic field can promote the growth of hair in the growth cycle of mice. In this process, the low frequency electromagnetic field promoted the expression of PDGF-BB and FGFR2 in KGF- 尾 1.
【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R454.1

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