海南省12株脑膜炎奈瑟菌分型与耐药性分析

发布时间：2018-01-16 03:28

本文关键词：海南省12株脑膜炎奈瑟菌分型与耐药性分析　出处：《现代预防医学》2017年10期 　论文类型：期刊论文

【摘要】：目的了解海南省脑膜炎奈瑟菌菌型和对抗生素的敏感性,为临床治疗和流脑防治提供科学依据。方法用玻片凝集法和聚合酶链反应(PCR)对本实验室2010-2015年收集的分离自病人和健康带菌者菌株进行血清分型和基因分群;用药敏纸片法和E-test试纸条法进行14种抗生素敏感性试验。结果共有12株脑膜炎奈瑟菌,其中10株血清分型结果与基因分群结果一致,2株血清不可分群菌株经PCR检测为C群和B群。3株患者分离株中,C群2株,B群1株。9株健康者分离株基因分群为B群3株,W135群、Y群各2株,C群和不可分群各1株。所有菌株对氨苄西林、头孢噻肟、头孢曲松、美罗培南、氯霉素、利福平、阿奇霉素、米诺环素均敏感。1株C群患者菌株对青霉素敏感性降低。83.3%(10/12)菌株对磺胺和复方新诺明均耐药。对环丙沙星、萘啶酸、左氧氟沙星的耐药率分别为66.7%(8/12)、58.3%(7/12)、25%(3/12)。结论海南省2010-2015年流脑的流行菌群是C群和B群,健康带菌者以B群、W135群和Y群为主。大部分菌株对复方新诺明、环丙沙星等磺胺类和喹诺酮类药物耐药。
[Abstract]:Objective to investigate the types of Neisseria meningitidis and the susceptibility to antibiotics in Hainan Province. Methods the slide agglutination method and polymerase chain reaction (PCR) were used to provide scientific basis for clinical treatment and prevention and treatment of meningitis. Serotyping and genotyping of strains isolated from patients and healthy carriers collected in our laboratory from 2010 to 2015; Results there were 12 strains of Neisseria meningitidis, and 10 of them had the same results as the genotyping results. The gene subgroups of 2 serum-indistinguishable strains were identified by PCR as group C and group B, respectively. 2 strains of group C and 1 strain of group B and 9 strains of healthy subjects were subdivided into group B and group B, respectively. All strains were ampicillin, cefotaxime, ceftriaxone, meropenem, chloramphenicol, rifampicin, azithromycin. Minocycline sensitivity. 1 strains of group C patients were less sensitive to penicillin. 83.3% 10 / 12) strains were both resistant to sulfanilamide and compound sulfamethoxine, to ciprofloxacin and naphthalic acid. The drug resistance rates of levofloxacin were 66.7% and 58.3%, respectively. Conclusion from 2010 to 2015 in Hainan Province, the epidemic flora of meningitis is group C and B, and the healthy carriers are group B. Most of the strains were resistant to sulfanilamides and quinolones, such as compound sulfamide and ciprofloxacin.
【作者单位】：海南省疾病预防控制中心检验检测所微生物检验科;
【基金】：海南省自然科学基金(No.814339) 海南省卫计委资助项目(No.琼卫2013资助-057号) 国家科技重大专项课题(2013ZX10004-203-002-006)
【分类号】：R440
【正文快照】： 流行性脑脊髓膜炎(Epidemic CerebrospinalMeningitis,ECM)简称流脑,是由脑膜炎奈瑟菌(Neisseria meningitidis,Nm)感染脑膜或脑脊髓膜引起的急性呼吸道传染病,常在冬春季节发病和流行[1]。脑膜炎奈瑟菌有A、B、C、H、I、K、L、W135、X、Y、Z、29E等12个荚膜多糖血清群,其中A

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