铜绿假单胞菌脂多糖对白色念珠菌生物膜的影响

发布时间：2018-01-16 08:25

本文关键词：铜绿假单胞菌脂多糖对白色念珠菌生物膜的影响　出处：《首都医科大学学报》2017年04期 　论文类型：期刊论文

【摘要】：目的研究铜绿假单胞菌脂多糖(lipopolysaccharide,LPS)对白色念珠菌生物膜形成的抑制作用。方法甲基四氮盐[(2,3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide,XTT]减低法用于检测铜绿假单胞菌LPS对白色念珠菌生物膜形成不同阶段生成量的影响,同时,利用倒置显微镜观察生物膜形态学改变;实时定量聚合酶链式反应(quantitative real time polymerase chain reaction,qRT-PCR)法检测铜绿假单胞菌LPS对白色念珠菌生物膜菌丝特异基因(hypha specific genes,HSGs)表达的影响。结果铜绿假单胞菌LPS可以抑制白色念珠菌生物膜的形成,以及使菌丝特异基因HWP1、ALS1、ALS3、ECE1和SAP4的表达分别下调8.3~12.8、1.1~3.0、2.0~4.6、1.3~3.8和6.2~7.6倍。结论铜绿假单胞菌LPS可以通过抑制白色念珠菌菌丝的形成从而抑制其生物膜的形成。
[Abstract]:Objective to study the inhibitory effect of lipopolysaccharide from Pseudomonas aeruginosa on biofilm formation of Candida albicans. [2. 2-methoxy-4-nitro-5-sulphophenyl). -2H-tetrazolium-5-carboxanilide. XTT was used to detect the effect of Pseudomonas aeruginosa LPS on the formation of Candida albicans biofilm at different stages, and the morphological changes of biofilm were observed by inverted microscope. Quantitative real time polymerase chain reaction. The specific gene hypha specific genes of Pseudomonas aeruginosa LPS against Candida albicans biofilm was detected by qRT-PCR. Results Pseudomonas aeruginosa LPS could inhibit the formation of biofilm of Candida albicans and induce the hyphae specific gene HWP1ALS1 and ALS3. The expression of ECE1 and SAP4 were down-regulated by 8.3 ~ 12.8 ~ 1.1 ~ 3.0 ~ 2.0 ~ 4.6, respectively. Conclusion Pseudomonas aeruginosa LPS can inhibit the formation of biofilm by inhibiting the formation of Candida albicans hyphae.
【作者单位】：首都医科大学附属北京友谊医院临床检验中心;
【分类号】：R446.5
【正文快照】： 白色念珠菌能够黏附在导尿管、心脏支架、静脉插管、人工关节等人体植入物上形成生物膜,从而对机体造成损害及对抗真菌药物产生明显耐药性[1]。因此,减少或阻断生物膜的形成可以有效提高抗真菌治疗效果,提高病人生存率。铜绿假单胞菌是人体常见的机会致病菌,也是医院获得性血

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