高频超声心动图评价心梗小鼠左室整体功能和节段性室壁运动异常

发布时间：2018-01-16 18:40

本文关键词：高频超声心动图评价心梗小鼠左室整体功能和节段性室壁运动异常　出处：《南方医科大学学报》2017年08期 　论文类型：期刊论文

【摘要】：目的通过建立小鼠心梗模型,探讨应用超高分辨率超声系统评价心梗小鼠心脏结构及心功能方面的应用价值。方法 25只雄性C57BL/6小鼠随机分为2组,即开胸结扎左冠状动脉前降支建立心梗模型组(n=15)和仅在相同结扎位置穿针,不结扎的方法建立假手术组(n=10)。术前3 d和术后1周采用二维、M型、脉冲多普勒(PWD)、组织多普勒(TDI)和EKVTM超声技术对心梗小鼠心脏形态结构、室壁运动以及心功能进行评价。术后1周,取小鼠心脏进行HE染色并观察心肌组织结构。结果二维超声与HE色染均显示,心梗模型组心肌有明显的梗死区;心梗模型组与假手术组比较,心室明显扩大,室壁明显变薄,心室收缩功能与舒张功能均显著下降(P0.05);局部室壁运动受阻严重,心室发生重构。结论采用2D超声心动图联合M型、PWD、TDI以及EKVTM超声心动图观察小鼠心梗模型能够更精准敏感地判断心肌梗死位置和梗死程度,为临床心肌梗死诊断、治疗和预后评估提供了参考价值。
[Abstract]:Objective to establish myocardial infarction model in mice. To evaluate the value of ultra-high resolution ultrasound system in evaluating cardiac structure and cardiac function in myocardial infarction mice. Methods 25 male C57BL / 6 mice were randomly divided into two groups. The left anterior descending coronary artery was ligated by thoracotomy in the myocardial infarction model group (n = 15) and the needle was punctured only in the same ligation position. The method of non-ligation was established in the sham operation group (n = 10). Two dimensional M type, pulse Doppler Doppler PWD was used 3 days before operation and 1 week after operation. Tissue Doppler technique (TDI) and EKVTM were used to evaluate the cardiac morphology, wall motion and cardiac function of myocardial infarction mice. Results Two-dimensional ultrasound and HE staining showed that myocardial infarction area was obvious in myocardial infarction model group. Compared with the sham operation group, the myocardial infarction model group showed that the ventricle enlarged obviously, the ventricular wall became thinner, and the systolic and diastolic function of the ventricular decreased significantly (P 0.05). Conclusion 2D echocardiography combined with M-type PWD is used. TDI and EKVTM echocardiography can more accurately and sensitively judge the location and degree of myocardial infarction, and provide reference value for clinical diagnosis, treatment and prognosis evaluation of myocardial infarction.
【作者单位】：广东省实验动物监测所//广东省实验动物重点实验室;广东药科大学;广州医科大学;复旦大学附属中山医院;四川省医学科学院四川省人民医院麻醉科;
【基金】：国家自然科学基金(31672376) 广东省科技专项(2016A020216019) 国家自然科学基金-青年科学基金(81500281) 广东省实验动物重点实验(2013A061402001)~~
【分类号】：R540.45;R542.22
【正文快照】： 人民医院麻醉科,四川成都610072High-frequency echocardiography for assessment of regional wall motion abnormalityand cardiac function in mice with myocardial infarctionWANG Jing1,TAN Weijiang1,LI Xiang1,2,ZHANG Guiping3,HUANG Jiayuan4,CHEN Xiaohong1,LEI Qi

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