血小板聚集率与富血小板血浆血小板浓度关系的研究

发布时间：2018-01-19 21:33

本文关键词： 血小板聚集率富含血小板血浆血小板浓度二磷酸腺苷花生四烯酸　出处：《中国实验血液学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨富血小板血浆(PRP)中血小板浓度对血小板聚集率的影响,初步确定诱聚剂二磷酸腺苷(ADP:5.0和15μmol/L)和花生四烯酸(AA:500.0μg/ml)对血小板浓度的允许范围。方法:选取健康查体人群枸橼酸钠抗凝外周血标本72例,每12例标本混合为1组,分6组。将各组标本混合后,分别制备不同水平的血小板浓度,利用海伦娜血小板聚集分析仪分别检测AA和ADP诱导下的6组不同血小板浓度水平的血小板聚集率。结果:当诱聚剂浓度升高,不同浓度的血小板聚集率会随之增高。随着血小板浓度升高,AA和ADP(15μmol/L)诱导的血小板聚集率均呈现先快速上升而后平缓的趋势,其中当Plt浓度低于200×10~9/L时,AA诱导的血小板聚集率随着血小板浓度的降低出现陡降,而ADP诱导的血小板聚集率随着血小板浓度的升高稍平缓些。当Plt200×10~9/L,AA 500μg/ml和ADP 15μmol/L诱导时,不同浓度Plt的血小板聚集率变化平缓、波动较少,较稳定。结论:进行血小板聚集率检测时,AA和ADP诱聚剂对应的适宜Plt浓度应大于200×10~9/L。
[Abstract]:Objective: to investigate the effect of platelet concentration on platelet aggregation in platelet-rich plasma (PRP). ADP: 5.0 and 15 渭 mol / L) and AA: 500.0 渭 g / ml). Methods: 72 cases of peripheral blood samples with sodium citrate anticoagulant were selected. Every 12 samples were mixed into 1 group and divided into 6 groups. After each group was mixed, different levels of platelet concentration were prepared. The platelet aggregation rates of 6 groups induced by AA and ADP were measured by Helena platelet aggregation analyzer. Results: when the concentration of attractant increased. With the increase of platelet concentration, the platelet aggregation rate induced by AA and ADP(15 渭 mol / L increased rapidly first and then slowly. When the concentration of Plt was lower than 200 脳 10 ~ (9) / L, the platelet aggregation rate induced by ACA decreased sharply with the decrease of platelet concentration. However, the platelet aggregation rate induced by ADP was slightly slower with the increase of platelet concentration, when Plt200 脳 10 ~ (-1) / L was used. When AA 500 渭 g / ml and ADP 15 渭 mol/L were induced, the platelet aggregation rate of different concentrations of Plt changed slowly and fluctuated less. Conclusion: the suitable concentration of Plt corresponding to AA and ADP should be more than 200 脳 10 ~ (9) L ~ (-1) for platelet aggregation.
【作者单位】：中国人民解放军总医院海南分院内分泌科;海南医学院热带医学与检验医学院;解放总医院海南分院检验中心;
【基金】：解放军总医院科技创新苗圃基金(16KMM43)
【分类号】：R457.1
【正文快照】： 血小板聚集实验(platelet aggregation test,PAgT)对早期发现是否有血栓形成危险以及阐明相关疾病的机制等有重要意义。我国血栓性疾病的发病率和死亡率位居其他疾病之首,在美国血栓性疾病的死亡率是肿瘤死亡率的5倍多,严重危害人类健康[1-2]。大量研究表明,糖尿病、高血

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