气泡微萃取-气相色谱-质谱法测定尿中咖啡因

发布时间：2018-01-22 06:45

本文关键词： 气泡微萃取咖啡因气相色谱/质谱尿　出处：《分析化学》2017年05期 　论文类型：期刊论文

【摘要】：建立了气泡微萃取结合气相色谱/质谱技术(GC-MS)测定尿中咖啡因的方法。对影响萃取效率的实验条件进行了优化,确定了最佳萃取条件:三氯甲烷作为萃取溶剂,萃取溶剂暴露体积1μL,气泡体积1.6μL,搅拌速度300 r/min,萃取时间5 min,盐度15%(m/V),气泡与磁子间距离1 cm。在优化条件下,所建立方法在咖啡因浓度0.005~10 mg/L范围内有较好的线性关系,相关系数可达0.986,检出限为0.003 mg/L。在人尿液中添加不同浓度的咖啡因(0.050、0.500和5.000 mg/L),回收率为89.2%~107.5%,相对标准偏差小于8%(n=6)。
[Abstract]:A method for determination of caffeine in urine by gas chromatography / mass spectrometry (GC / MS) with bubble microextraction was established and the experimental conditions affecting the extraction efficiency were optimized. The optimum extraction conditions were determined as follows: trichloromethane as extraction solvent, solvent exposure volume 1 渭 L, bubble volume 1.6 渭 L, stirring speed 300r / min, extraction time 5 min. Under the optimized conditions, the established method has a good linear relationship in the range of 0.005 ~ 10 mg/L of caffeine concentration, and the distance between the bubble and the magneton is 1 cm 路m ~ (-1), the salinity is 15 cm / V ~ (-1), and the distance between the bubble and the magneton is 1 cm. The correlation coefficient was 0.986, the detection limit was 0.003 mg / L. Different concentrations of caffeine were added to human urine (0.050 mg / L and 5.000 mg / L). The recovery was 89. 2% and 107.5%, and the relative standard deviation was less than 8%.
【作者单位】：甘肃政法学院公安技术学院;兰州军区农业新技术试验培训基地;
【基金】：国家自然科学基金(No.21565005) 甘肃省自然科学基金(No.1506RJZA033) 兰州市科技计划(No.2016-3-36)资助项目~~
【分类号】：R446.12;O657.63
【正文快照】： 1引言咖啡因(Caffeine)是一种嘌呤类生物碱,即1,3,7-三甲基黄嘌呤,属中枢神经系统兴奋剂,适量摄入能够缓解疲劳、振奋精神、提高注意力[1,2,],被广泛用作食品添加剂[3]。同时,咖啡因也是一种毒品,不当使用会造成上瘾、神经过敏和睡眠紊乱等不良反应,严重者甚至会导致中毒或死

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