SCSA和SCD检测精子DNA完整性结果比较及与精子质量参数的相关性分析

发布时间：2018-01-22 17:06

本文关键词： 精子DNA碎片精子染色质结构分析试验精子染色体扩散实验精子活动率正常形态精子百分率年龄　出处：《中华男科学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:近年来精子DNA碎片预测男性生育受到广泛关注,其检测方法较多,但方法学的比较研究很少,本文旨在比较精子DNA完整性常用检测方法之间的差异,并分析DNA碎片与精子质量的相关性。方法:选择108例精液样本,采用精子染色质结构分析试验(SCSA)和精子染色体扩散实验(SCD)对样本分别进行精子DNA碎片检测分析,对两种方法的结果进行比较,并与精液常规、精子形态以及患者年龄进行相关性分析。结果:两种方法检测的精子DNA碎片化指数(DFI)存在显著的一致性(P0.01),DFI与精子活动率、前向运动精子百分率以及正常形态精子百分率呈显著负相关(P0.01),与畸形精子指数呈显著正相关(P_(SCSA)0.01,P_(SCD)0.05),SCSA得出的DFI与年龄呈显著正相关(P0.01),而SCD法的DFI并未发现类似的现象。结论:两种方法检测的结果对精子质量均有较好的预测价值,精子DFI作为一个检测指标,对男性生育力预测有一定的临床价值。但不同方法之间的检测结果差异较大,检测方法的标准化有待进一步研究。
[Abstract]:Objective: in recent years, sperm DNA fragments have attracted wide attention in predicting male fertility, and there are many methods for their detection, but there are few comparative studies on the methods. The aim of this study was to compare the differences of sperm DNA integrity test methods and to analyze the correlation between DNA fragments and sperm quality. Methods: one hundred and eight semen samples were selected. Sperm chromatin structure analysis test (SCSA) and sperm chromosome diffusion test (SCD) were used to detect and analyze sperm DNA fragments, and the results of the two methods were compared. The correlation analysis was made with semen routine, sperm morphology and patient's age. Results: there was significant consistency between the two methods in detecting sperm DNA fragmentation index (P 0.01). DFI was negatively correlated with sperm motility, forward motility and normal sperm morphology (P0.01). There was a significant positive correlation between DFI and age (P < 0.01). But the DFI of SCD method did not find similar phenomenon. Conclusion: the results of the two methods have a good predictive value for sperm quality, sperm DFI as a detection index. It has some clinical value to predict male fertility, but the results of different methods are quite different, and the standardization of test methods needs further study.
【作者单位】：南京医科大学附属苏州市立医院生殖与遗传中心;
【基金】：国家自然科学基金(81170611) 江苏省自然科学基金(BK2012601) 江苏省遗传医学重点学科基金(FXK201305) 苏州市临床重点病种诊疗技术专项(lczx201411) 苏州市临床医学中心(szzx201505)~~
【分类号】：R698.2;R446.1
【正文快照】： Supported by grants from National Natural Science Foundation of China(81170611),Natural Science Foundation of Jiangsu Province(BK2012601),Key Discipline Foundation for Medical Genetics of Jiangsu Province(FXK201305),Specialized Fund for Diagnosis and Tre

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