荧光定量PCR检测献血人员外周血白细胞中人疱疹病毒DNA

发布时间：2018-01-23 12:47

本文关键词： 人疱疹病毒血源性感染荧光定量PCR 分子检测　出处：《安徽医科大学学报》2017年09期 　论文类型：期刊论文

【摘要】：目的调查献血人员外周血白细胞中8种人疱疹病毒的DNA分布情况,为我国献血者健康标准要求的完善提供参考性实验数据。方法收集献血人员外周血标本427例,分离外周血白细胞后提取DNA,使用Taqman探针法荧光定量PCR检测方法对8种疱疹病毒DNA的阳性率和病毒载量进行检测。结果 427例标本中未检测到单纯疱疹病毒1型(HSV1)、HSV2、水痘-带状疱疹病毒(VZV)和人疱疹病毒8型(HHV-8)的DNA,EB病毒(EBV)、人巨细胞病毒(HCMV)、HHV-6、HHV-7阳性率分别为51.4%、6.5%、50.6%、21.1%,其中HCMV、EBV和HHV-6均阳性的标本占6.5%,EBV和HHV-6均阳性的标本占25.1%,EBV与HHV-7均阳性的标本占4.0%,HHV-6和HHV-7均阳性的标本占6.9%。EBV、HCMV、HHV-6、HHV-7的病毒载量中位数分别为559、336、4 683、1 126 gqe/ml外周血。在不同性别和年龄组中EBV、HHV-6和HHV-7在三个年龄组中的检出率差异无统计学意义,但CMV的检出率随年龄的增加逐渐升高,差异有统计学意义(P0.05)。市区与郊区人群相比EBV、HCMV、HHV-6和HHV-7的检出率均显著较低(OR=0.657、0.798、0.889、0.721,95%CI:0.147~2.451、0.224~2.196、0.221~2.703、0.194~2.445,P0.05)。结论在献血人员外周血白细胞中可以检出多种疱疹病毒DNA,提示我国献血者健康标准要适时考虑献血者疱疹病毒感染的情况。
[Abstract]:Objective to investigate the DNA distribution of eight human herpesviruses in peripheral white blood cells (PBWs) of blood donors. Methods the peripheral blood samples of 427 blood donors were collected and DNA was extracted from peripheral white blood cells. The positive rate and viral load of 8 herpesvirus DNA were detected by Taqman probe fluorescence quantitative PCR method. Results No herpes simplex virus type 1 was detected in 427 samples. HSV1). The DNA of HSV2, varicella-zoster virus (VZV) and human herpesvirus 8 (HHV-8) were detected by EBV. The positive rate of HHV-7 was 51.4 / 6.5 and 50.6 / 21.1respectively, of which 6.5% were positive for both HHV-7 and HHV-6. The positive specimens of EBV and HHV-6 were found to be positive for EBV and HHV-7 in 25.1. the positive specimens of HHV-6 and HHV-7 were 6.9. EBV. The median viral load of HHV-6 and HHV-7 of HCMV was 559 ~ 336n ~ 4 683, respectively. 1 126 gqe/ml peripheral blood. There was no significant difference in the detection rate of EBV HHV-6 and HHV-7 among the three age groups in different sex and age groups. But the detection rate of CMV gradually increased with the increase of age, the difference was statistically significant (P 0.05). The detectable rates of HHV-6 and HHV-7 were significantly lower than that of ORX 0.657U 0.798U 0.889 / 0.721 / 95 CI: 0.147 / 2.451. 0.224c2.196 / 0.221 / 2.703 / 0.194 / 2.445. Conclusion A variety of herpesvirus DNA can be detected in peripheral white blood cells of blood donors, which suggests that the situation of herpesvirus infection in blood donors should be taken into account in the health standard of blood donors in China.
【作者单位】：安徽医科大学微生物学教研室;无锡市红十字中心血站检验科;无锡市第二人民医院检验科;
【基金】：国家自然科学基金(编号:81571963)
【分类号】：R440
【正文快照】： 2017-05-03接收人疱疹病毒(human herpesviruses,HHVs)分为α、β、γ三个亚科。α亚科包括单纯疱疹病毒1型(herpes simplex virus 1,HSV1)、HSV2和水痘-带状疱疹病毒(varicella-zoster virus,VZV);β亚科包括人巨细胞病毒(human cytomegalovirus,HCMV)、HHV-6和HHV-7;γ亚科

【参考文献】