基于gyrB基因建立快速检测沙雷菌的PCR方法

发布时间：2018-01-24 06:56

本文关键词： 沙雷菌肠道菌 gyrB基因 PCR　出处：《畜牧与兽医》2017年07期 　论文类型：期刊论文

【摘要】：选择gyr B基因作为靶基因设计特异性引物用于沙雷菌属的PCR检测,该特异性引物扩增产物的片段大小为279 bp。研究所选用的14株沙雷菌分别代表沙雷菌属的7个不同种。此外,2种沙门菌、2种克雷伯菌以及其他5种肠道菌用于进行引物特异性的验证。结果表明:扩增的条带与预期的扩增产物片段大小一致,而非沙雷菌属的其他9个种属均没有扩增条带;该方法检测沙雷菌的最低检测限为9.785 pg。通过对4株从蜜蜂中分离所得的黏质沙雷菌和3株从进口鱼粉中分离所得的居泉沙雷菌进行检测,结果均为阳性。研究表明,基于gyr B基因建立的PCR方法在沙雷菌属的检测中具有特异性强、快速和灵敏度高等特点。
[Abstract]:Gyr B gene was selected as the target gene to design specific primers for PCR detection of the genus Shareh. The fragment size of the amplified product was 279 BP. The 14 strains of Shareh bacteria represented 7 different species of Shareh respectively. In addition, two Salmonella species were selected. Two Klebsiella species and five other enterobacteria were used to verify the primer specificity. The results showed that the amplified bands were the same as the expected product size. But none of the other 9 species of the genus Shareh had amplified bands. The minimum detection limit of this method was 9.785 PG. Four strains of Shareh isolated from bees and three strains of Shareh isolated from imported fish meal were detected. The results showed that the PCR method based on gyr B gene was specific, rapid and sensitive in the detection of the genus Shareh.
【作者单位】：福建农林大学/福建省兽医中药与动物保健重点实验室;福建农林大学生命科学学院;福建出入境检验检疫局/福建省检验检疫技术研究重点实验室;
【基金】：国家自然科学基金(31272606) 国家质检总局科技计划项目(2016IK030) 福建检验检疫局科技计划项目(FK2015-JS002)
【分类号】：R446.5
【正文快照】： 沙雷菌在分类上属肠杆菌科(Enterobacteriaceae)沙雷菌属(Serratia)。沙雷菌属现有包括黏质沙雷菌(Serratia marcescens)、居泉沙雷菌(Serratiafonticola)、液化沙雷菌(Serratia liquefaciens)等14个种,2个亚种[1]。沙雷菌是一种广泛分布于水、土壤、植物等多种自然环境以及人

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